TLR_4及其配体对矽肺晚期炎症的作用研究  

Effects of TLR_4 and Its Ligands on Late Silicosis Inflammation

作　　者：万文娟 张建斌 王晓云 李素平[2] 

机构地区：[1]长治医学院公共卫生与预防医学系,046000 [2]山西医科大学公共卫生学院

出　　处：《长治医学院学报》2017年第4期241-243,共3页Journal of Changzhi Medical College

基　　金：山西省自然科学基金(2011011035-3)

摘　　要：目的:从细胞水平初步探讨TLR_4及其配体对矽肺晚期炎症的作用。方法:(1)制备小鼠矽肺模型:染尘采用滴鼻注入SiO_2悬液方法,剂量为200mg/kg,每只注入量为0.1mL。对照组在相同条件下滴鼻注入生理盐水0.1mL。(2)指标检测:Western blot检测成纤维细胞内TLR_4和RAGE蛋白含量。结果:(1)TLR_4结果显示:TLR_4表达水平随观察时间的延长明显升高;(2)RAGE结果显示:RAGE在正常对照组呈现高表达,在3d染尘时段表达量最高且均高于正常对照组,其余染尘组表达量降低。结论:在矽肺晚期炎症的形成过程中,HMGB1发挥生物学效应不完全依赖RAGE受体,还有TLR_4的参与。Objective：To explore the effects of TLR_4 and its ligands on late silicosis from the cell level.Methods：（1）Silicosis model preparation：SiO_2 suspension was injected with the method of nasal drip,with a dose of 200 mg/kg,each injection volume of 0.1mL.The control group was injected saline solution of 0.1mL under the same condition.（2）Index detection：The content of TLR_4 and RAGE were detect by Western blot.Results：（1）TLR_4 results showed that the expression level of TLR_4 was significantly increased with the prolongation of observation time（P0.05）.（2）The results of RAGE showed that RAGE presented high expression in the normal control group;and the expression level was the highest in the three days of dusting day while the expression of the remaining dusting groups decreased（P0.05）.Conclusion：In the formation of late silicosis inflammation,the biological effects of HMGB1 do not rely solely on the RAGE receptor,but also on the participation of TLR_4.

关 键 词：高迁移率族蛋白1 晚期糖基化终末产物受体 TOLL样受体4 肺成纤维细胞 

分 类 号：R563.9[医药卫生—呼吸系统]