基于石墨烯信号放大策略的胆固醇分子印迹电化学传感器研究  

Molecularly Imprinted Electrochemical Sensor for Determination of Cholesterol Based on Signal Amplification of Graphene

作　　者：白慧萍[1,2] 王春琼 曹秋娥 

机构地区：[1]云南大学化学科学与工程学院,昆明650091 [2]云南大学材料科学与工程学院,云南省微纳材料与技术重点实验室,昆明650091 [3]云南省烟草质量监督检测站,昆明650106

出　　处：《分析化学》2017年第10期1535-1541,共7页Chinese Journal of Analytical Chemistry

基　　金：云南省教育厅科学基金(No.2017ZZX231);国家自然科学基金项目(Nos.21465025,21505119)资助~~

摘　　要：利用石墨烯的信号放大作用,结合分子印迹技术和电聚合技术,以苯酚为功能单体,胆固醇(ChO)为模板分子,在石墨烯修饰的玻碳电极表面电聚合能识别胆固醇分子的敏感膜,构建胆固醇分子印迹电化学传感器。采用扫描电镜、循环伏安法、差分脉冲伏安法研究了印迹膜的结构、性能和分子印迹效应。结果表明,此传感器对胆固醇有良好的选择性和较高的灵敏度。胆固醇浓度在8.0×10^(-8)~2.0×10^(-4)mol/L范围内与峰电流的变化呈线性关系,检出限(S/N=3)为5.6×10^(-8)mol/L。将其用于人体血清样品的检测,结果满意。An electrochemical sensor was fabricated for determination of cholesterol（ChO） based on the molecularly imprinting and signal amplification of graphene.The sensor was prepared by electropolymerizing phenol on the surface of graphene modified glassy carbon electrode with ChO as template.The structure,properties and molecular imprinting effect of the imprinted membrane were studied by SEM,CV and DPV.The results showed that the sensor had good selectivity and high sensitivity to cholesterol.The calibration graph for the determination of cholesterol was linear in the range of 8.0×10^（-8）-2.0×10^（-4）mol/L,with the detection limit（S/N=3） of 5.6×10^（-8）mol/L.The sensor was applied to the determination of cholesterol in human serum samples with satisfactory results.

关 键 词：石墨烯 胆固醇 分子印迹膜 电化学传感器 

分 类 号：O657.1[理学—分析化学] TP212[理学—化学]