介孔沸石材料负载传统基质用于激光解吸电离-飞行时间质谱分析小分子化合物  

Analysis of Small Molecules by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry with Porous Zeolite Supported α-Cyano-4-Hydroxycinnamic Acid as Composite Matrix

作　　者：杨梦瑞[1] 李鹏[1] 王敏[1] 周剑[1] 刘芳[1] 张丽媛[1] 

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,农产品质量与食物安全重点开放实验室,北京100081

出　　处：《分析化学》2017年第10期1542-1546,共5页Chinese Journal of Analytical Chemistry

基　　金：中国农业科学院基本科研业务费专项(No.1610072016010);中国农业科学院科技创新工程项目资助~~

摘　　要：考察了介孔沸石材料负载传统有机基质α-氰基-4-羟基桂皮酸(CHCA)用于基质辅助激光解吸电离-飞行时间质谱(MALDI-TOF-MS)分析多肽Substance P和氟喹诺酮类药物等小分子的效果。在相同的MALDI-TOF-MS质谱条件下,与传统CHCA进行了比较,同时分别考察了不同硅铝比(SiO_2/Al_2O_3)的ZSM-5以及不同介孔大小的Beta与ZSM-5沸石载体对Substance P的检测效果。结果表明,沸石负载CHCA新型复合基质具有抑制碱金属离子峰、消除干扰碎片离子、简化与改善质谱图、提高离子化效率等优点。实验结果表明,沸石表面酸性越强,有力介孔能够充分包裹CHCA分子,则复合基质抑制干扰碎片和提高离子化效率的能力越高。复合基质成功应用于复杂样品中恩诺沙星与诺氟沙星药物小分子的MALDI-TOF-MS检测。Zeolite supported α-cyano-4-hydroxycinnamic acid（CHCA） as novel composite matrix was applied in matrix assisted laser desorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS） analysis of substance P and small molecular compounds of fluoroquinolones.In the experiment,this new matrix was examined and compared with conventional organic matrix CHCA.The results indicated that the novel composite matrix was capable of suppressing the alkali metal related peaks and interference fragments,simplifying mass spectra in low-molecular-weight region,and promoting ionization efficiency.The influence of SiO_2/Al_2O_3 ratio of ZSM-5 and porous diameter of ZSM-5 and Beta zeolites on performance of MALDI-TOF-MS analysis of substance P was also investigated under the same conditions.In contrast,it was regarded that the strong surface acidity and sufficient cavity for loading CHCA were beneficial for interference fragments suppression and peak intensity enhancement.Additionally,it was successfully applied in the detection of enrofloxacin and ciprofloxacin in complex samples.

关 键 词：介孔沸石 激光解吸电离-飞行时间质谱 小分子化合物 氟喹诺酮类药物 

分 类 号：O657.63[理学—分析化学]