迷迭香乙酸乙酯萃取物对大鼠溃疡性结肠炎的保护作用研究  

Protective Effect of Ethyl Acetate Extract of Rosmarinus officinalis on Ulcerative Colitis in Rats

作　　者：胡还甫[1] 宋旭日[1] 刘刚[1] HU Huan-fu SONG Xu-ri LIU Gang(Key Laboratory of Comprehensive Utilization Characteristic Chinese Herbal Medicines of Dongting Lake, Yueyang Vocational Technical College, Yueyang , Hunan, 414000, China)

机构地区：[1]岳阳职业技术学院/洞庭湖特色中药材综合利用重点实验室,湖南岳阳414000

出　　处：《动物医学进展》2017年第10期69-72,共4页Progress In Veterinary Medicine

基　　金：湖南省教育厅资助项目(14C1156;16C1637)

摘　　要：为研究迷迭香乙酸乙酯萃取物对大鼠溃疡性结肠炎的保护作用机制,采用DSS建立大鼠溃疡性结肠炎模型,各组分别给予低、中、高剂量的迷迭香乙酸乙酯萃取物,连续给药2周,检测TLR4、TNF-α、MD2、IL-13、IL-8、IL-10水平及结肠组织病理学变化。结果显示,迷迭香乙酸乙酯萃取物中、高剂量能明显降低TLR4、TNF-α、MD2、IL-8水平,提升IL-13、IL-10水平(P<0.05),改善大鼠溃疡性结肠模型结肠病理改变。说明迷迭香乙酸乙酯萃取物对溃疡性结肠炎具有明显的保护作用。To study the protective effect of ethyl acetate extract of Rosmarinus officinalis on the model of ulcerative colitis, the model of ulcerative colitis was established by using DSS. The low, medium and high doses of ethyl acetate extracts of Rosmarinus officinalis were continuously administered to rats in each group for two weeks. The serum levels of TNF-α, IL-8 and histopathological lesions of colon were exam- ined. The results showed that medium and high doses of ethyl acetate extracts of Rosrnarinus officinalis significantly reduced TLR4, TNF-α, MD2, IL-8 levels, and increased IL-13, IL-10 （P〈0. 05）, and decreased the pathological lesions of the colon of ulcerative colitis rat model. It demonstrated that ethyl acetate extract of Rosrnarinus officinalis had a significant protective effect on ulcerative the colitis model.

关 键 词：迷迭香 乙酸乙酯萃取物 溃疡性结肠炎 

分 类 号：S852.374[农业科学—基础兽医学]