阜新某鸡场大肠埃希菌四环素类药物敏感性试验及耐药基因检测  

Tetracycline Sensitivity Test and Drug Resistance Gene Detection of Escherichia coli in One Chicken Farm of Fuxin

作　　者：张雅为[1] 高锋 刘耀川[3] 朱江巍[3] 魏澍[1] 王军[1] 赵培[1] 赵晓彤[1] 顾贵波[1] ZHANG Ya-wei GAO Feng LIU Yao-chuan ZHU Jiang-wei WEI Shu WANG Jun ZHAO PeiI ZHAO Xiao-tong GU Gui-bo(Animal Disease Prevention and Control Center in Liaoning Province, Shenyang, Liaoning, 110164, China Liaoning A grcultural Technical College , Yingkou ,Liaoning , 115009, China Major Animal Epidemic Emergency Center in Liaoning Province, Shenvang , Liaoning, 310161, China)

机构地区：[1]辽宁省动物疫病预防控制中心,辽宁沈阳110164 [2]辽宁农业职业技术学院,辽宁营口115009 [3]辽宁省重大动物疫病应急中心,辽宁沈阳110161

出　　处：《动物医学进展》2017年第10期118-121,共4页Progress In Veterinary Medicine

基　　金：辽宁省农业领域青年科技创新人才培养计划项目(2015015)

摘　　要：大肠埃希菌能够引起鸡大肠杆菌病,对养殖业危害巨大。检测阜新某鸡场分离的33株鸡源大肠埃希菌对6种临床常用的四环素类药物的敏感性,并对四环素耐药基因进行检测。结果表明,大肠埃希菌分离株对土霉素和脱氧土霉素高度耐药;对四环素和多西环素中度耐药;对金霉素和强力霉素敏感。在33株大肠埃希菌分离株中检测出tetB、tetC、tetM、tetK、tetL 5种四环素耐药基因,未检出tetA基因,tetK检出率最高。E. coli could cause chicken colibacilosis and bring huge harm to breeding industry. In this study, 33 E. coli isolates were obtained from a chicken breeding farm in Fuxin area, and antibiotic sensitivity test was taken out to detect the sensitivity of isolates to 6 kinds of tetracyclines which commonly used in clinical treatments. And drug resistance genes were screened. The results showed that, isolates were highly resist- ant to oxytetracycline and doxycyclinum, moderately resistant to tetracycline and doxycylcline, and sensitive to duomycin and deoxytetracycline. 5 kinds of resistance genes were screened in 33 E. coli isolates, the positive genes were tetB,tetC,tetM,tetK and tetL,none isolates was positive for tetA gene,and tetK was the major resistance gene.

关 键 词：鸡大肠埃希菌 四环素类 耐药 基因 

分 类 号：S852.612[农业科学—基础兽医学] S858.31[农业科学—兽医学]