基于响应面法优化玉蝉花切花保鲜剂配方的研究  

Optimization of Preservative Agent of Iris ensata Cut Flowers Based on Response Surface Methodology

作　　者：于晓萌[1] 杜钰 郝宏娟[1] 王玲[1] YU Xiaomeng DU Yul HAO Hongjuan WANG Ling(The college of Landscape, Northeast Forestry University, Harbin 150040, China)

机构地区：[1]东北林业大学园林学院,哈尔滨150040

出　　处：《中国农业科技导报》2017年第10期121-131,共11页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(31670344)资助

摘　　要：为延长玉蝉花切花的观赏时间和提高观赏品质,在对植物激素、有机酸和无机盐单因素试验的基础上进行Box-Behnken中心组合试验设计,并通过响应面回归分析优化玉蝉花切花组合保鲜剂配方。结果表明,最佳组合保鲜剂配方为30 mg/L蔗糖+250 mg/L 8-HQ+10.66 mg/L 6-BA+108.95 mg/L CA+909.95 mg/L CaCl_2,按此条件进行验证,测得玉蝉花切花瓶插寿命为6.8 d,较对照延长3.7 d;该处理还可增加切花花枝鲜质量,缓解切花花瓣中可溶性蛋白质的降解速度,提高其超氧化物歧化酶(SOD)活性,延缓丙二醛(MDA)含量的升高,本保鲜剂配方对于玉蝉花切花的推广有着重要的实践意义。To extend the view and admire time and improve visual quality of Iris.ensata cut flowers,Box-Behnken center-united experiment was designed on the basis of single-factor test including plant hormone,organic acid and mineral salt.Then the response surface regression analysis was conducted to optimize preservative formula for I.ensata cut flowers.The results showed that the optimal preservative formula was 30 mg/L sugar ＋ 250 mg/L 8-HQ ＋ 10.66mg/L 6-BA＋108.95 mg/L CA ＋ 909.95 mg/L CaCl2.Under these conditions,the cut flower life were 6.8 d,3.7 d longer than that of the control group.Meanwhile,this treatment could increase the fresh weight of flower stem,remit the degradation speed of soluble protein in cut flower petal,improve SOD activity,delay the increase of MDA.This preservative formula had important practical significance for the extension of I.ensata cut flowers.

关 键 词：玉蝉花切花 保鲜剂优化 延缓衰老 

分 类 号：S682[农业科学—观赏园艺]