哈萨克羊GDF9、ESR、BMPR-IB基因的PCR-RFLP多态性研究  

PCR-RFLP Polymorphisms of GDF9,ESR,BMPR-IB Genes in Kazakh Sheep

作　　者：路立里[1] 韩冰 陈春华 刘宜勇 张宁 Lu Lilil Hart Bing Chen Chunhua et al(Xinjiang Academy of Animal Sciences, Urumchi 830000, China Key Laboratory of Genetics, Breeding and Reproduction of Grass Feeding Livestock, Ministry ofAgricuhure, Urumchi 830000, China The Livestock Improvement Station of Yili State, Yining 835000, Xinjiang, China)

机构地区：[1]新疆畜牧科学院,乌鲁木齐830000 [2]农业部草食家畜遗传育种与繁殖重点实验室,乌鲁木齐830000 [3]新疆伊犁州畜禽改良站,伊宁835000

出　　处：《中国草食动物科学》2017年第5期6-9,共4页China Herbivore Science

摘　　要：为了探索绵羊多胎性的分子机理以及为绵羊繁殖力的标记辅助选择和育种提供理论依据,特开展了哈萨克羊品种多胎主效基因筛选和标记辅助育种研究。实验采用PCR-RFLP方法,对哈萨克羊GDF9、ESR、BMPR-IB基因进行PCR-RFLP多态性检测。结果表明:在哈萨克羊群体中GDF9、ESR、BMPR-IB基因的酶切位点上均未发现多态性,说明GDF9、ESR、BMPR-IB基因的酶切位点均不能作为控制哈萨克羊繁殖力的潜在分子标记位点。To explore the molecular mechanism of prolificacy and providing theoretical basis for marker-assisted selection and breeding of fecundity in sheep,PCR-RFLP was used to detect polymorphisms ofGDF9,ESRandBMPR-IBgenes in Kazakh sheep.control reproduction of Kazakh sheep.

关 键 词：哈萨克羊 GDF9基因 ESR基因 BMPR-IB基因 多态性 

分 类 号：S826.2[农业科学—畜牧学]