益气活血药物对脑梗死恢复期大鼠c-fos基因表达的影响  

Effect of Supplementing Qi and Activating Blood Circulation Drugs on C-Fos Mrna of Rats with Cerebral Infarction

作　　者：王嘉麟[1] 邢佳[1] 贺立娟[1] 朱晓晨[1] 王椿野[1] 王蕊 郑建颖[1] 张迎 李秋祥 崔洪志 秦裕驹 符海燕 高维 郭晓[1] 袁清洁 高东阳[1] 郭凯航 王建伟[1] 王玉来[1] 郭蓉娟[1] Wang Jialin Xing Jia He Lijuan Zhu Xiaochen Wang Chunye Wang Rui Zheng Jianying Zhang Ying Li Qiuxiang Cui Hongzhi Qin Yuju Fu Haiyan Gao Wei Guo Xiao Yuan Qingjie Gao Dongyang Guo Kaihang Wang Jianwei Wang Yulai Guo Rongjuan(Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China)

机构地区：[1]北京中医药大学东方医院,北京100078

出　　处：《世界中医药》2017年第9期2164-2167,共4页World Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)(2015CB554401);高等学校博士学科点专项科研基金资助课题(20120013120014);北京中医药大学东方医院人才培养计划项目(东方新星)(DFRC2014C01)

摘　　要：目的:基于c-fos mRNA观察益气活血药物对脑梗死恢复期大鼠的影响。方法:以Longa的方法略作改良制作大鼠左侧大脑中动脉阻塞模型,按照随机数字表的方法随机分为正常组、模型组、假手术组、益气组、活血组、益气活血组、ERK抑制剂7组,采用RT-PCR技术,在治疗28 d观察益气活血药物对脑梗死恢复期大鼠梗死皮层c-fos mRNA表达的影响。结果:在治疗28 d后,模型组较正常组c-fos mRNA表达明显下降(P<0.05),益气活血组与ERK抑制组较模型组有一定程度提高(P<0.05),活血组与模型组比较,无明显统计学意义(P>0.05),益气组的c-fos mRNA表达低于其他各组(P<0.05)。结论:在脑梗死恢复期,益气活血药物在一定程度上可改善脑缺血,优于单纯益气与单纯活血,其疗效机制可能与提高c-fos mRNA的表达有关。Objective： To observe the expression of c-fos mRNA of rats with cerebral infarction in recovery period under supplementing qi and activating blood circulation drugs. Methods： Longa method was slightly modified in making the rat model of left middle cerebral artery occlusion. According to the random number table method,the rats were randomly divided into 7 groups,including a normal group,a model group,a sham operation group,a supplementing qi group,an activating blood circulation group,a supplementing qi and activating blood circulation group,and an ERK inhibitor group. Using RT-PCR technique,we observed the effect of supplementing qi and activating blood circulation drugs on c-fos mRNA of rats with cerebral infarction after 28 days’ treatment in myocardial infarction cortex. Results： After 28 days’ treatment,compared with normal group,c-fos mRNA expression in the model group was significantly decreased（ P 〈 0. 05）; c-fos mRNA expression in the supplementing qi and activating blood circulation group and ERK inhibition group increased to a certain extent than that in the model group（ P 〈 0. 05）; the activating blood circulation group had no significant statistical difference with the model group（ P 〉 0. 05）; c-fos mRNA expression in supplementing Qi group was lower than the other groups（ P 〈 0. 05）. Conclusion： In the recovery period of cerebral infarction,supplementing qi and activating blood circulation drugs can improve cerebral ischemia in a certain degree,which is better than simply supplementing qi and activating blood circulation,and its therapeutic mechanism may be related to improving the expression of c-fos mRNA.

关 键 词：脑梗死 C-FOS 益气活血 

分 类 号：R256[医药卫生—中医内科学]