人尿中氟测定的离子色谱法  

Determination of fluorine-ion in human urine by ion chromatography method

作　　者：张福钢[1] 闫慧芳[1] 潘亚娟[1] 赵玮[1] Zhang Fugang Yan Huifang Pan Yajuan Zhao Wei.(National Institute of Occupational Health and Poison Control, China CDC, Beijing 100050, China)

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《中华劳动卫生职业病杂志》2017年第8期622-624,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家卫生计生委法制司卫生标准制定修订项目（20150712）;国家科技支撑计划课题（2014BAI12B00）

摘　　要：目的建立人尿中氟的离子色谱测定方法。方法样品经离心、稀释、C18固相萃取小柱处理后，以KOH为流动相梯度洗脱，经AS15柱分离，阴离子电导检测器检测。结果氟离子浓度的线性范围为0．02～10．00mg／L，相关系数大于0．999，方法检出限为0．06mg／L（以3ml尿计），加标回收率为96．9％-99．5％；批内精密度为0．40％～2．89％，批间精密度为1．01％～7．23％；尿样置于室温和4℃条件下能稳定保存7d，-20℃条件下可保存14d。结论该方法的各项指标均满足GBZ／T210．5．2008《职业卫生标准制定指南第5部分：生物材料中化学物质测定方法》的要求。Objective To establish a method to determine fluorine ion in human urine by ion chromatography method. Methods The samples were centrifuged, diluted and processed by C15 solid-phase column, potassium hydroxide as mobile phase, gradient elution,separated by AS15, and determined by anion conductivity detector. Results The linear correlation coefficient of fluoride ion in the range of 0.02-10.00 mg/L was more than 0.999. The lowest detection limit of urinary fluoride was 0.06 mg/L （in 3 ml urine）. The recoveries of the method were 96.9%-99.5%, the inter batch precision range was 0.40%-2.89%. The intra batch precision was from 1.01 to 7.23%. Fluoride samples could be stored for 7 days at room temperature and 4 ℃, and be stored for 14 days at -20 ℃. Conclusion Determination of fluoride in urine by ion chromatography ,the method could meet the requirement in GBZ/T 210.5-2008.

关 键 词：离子 色谱法 氟 尿 

分 类 号：R135[医药卫生—劳动卫生]