重组人脑钠尿肽对心力衰竭大鼠心肌重构及缝隙链接蛋白43表达的影响  

Effects of recombinant human brain natriuretic peptide on myocardial remodeling and connexin 43 expression in rats with heart failure

作　　者：谭文婷[1] 彭葳锐[1] 唐芬[1] 刘盛权 龙俊蓉 褚春 TAN Wenting PENG Weirul TANG Fen LIU Shengquan LONG Junrong CHU Chun(The First Affiliated Hospital of South China University, Hengyang 421001, China)

机构地区：[1]南华大学附属第一医院,湖南衡阳421001 [2]南华大学附属第二医院

出　　处：《山东医药》2017年第33期9-11,15,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81202830)

摘　　要：目的探讨重组人脑钠尿肽(rhBNP)对压力超负荷所致心力衰竭大鼠心肌重构及缝隙链接蛋白43(Cx43)表达的影响。方法将45只雄性SD大鼠随机分为心力衰竭(F)组、心力衰竭+rhBNP(B)组、假手术(SO)组各15只,F组、B组经腹主动脉缩窄法建立压力超负荷心力衰竭大鼠模型,SO组大鼠不缩窄腹主动脉。B组经腹腔皮下注射用生理盐水配制的rhBNP 25μg/(kg·d),SO组和F组仅用生理盐水腹腔皮下注射,均给药8周。检测各组左心功能、血流动力学、QT离散度、左室心肌组织病理形态、心肌纤维化沉积和心肌Cx43的表达。结果与SO组比较,F组左心功能、血流动力学、QT离散度、左室心肌组织病理形态、心肌纤维化沉积和心肌Cx43表达均有统计学差异(P均<0.05),显示心力衰竭模型制作成功。与F组比较,B组大鼠平均动脉压下降、QT离散度减小、心肌细胞排列紊乱程度减轻、心肌细胞间胶原纤维沉积减少、Cx43表达增加(P均<0.05)。结论rh BNP可改善压力超负荷下心力衰竭大鼠的血流动力学指标,上调心肌组织Cx43表达并抑制心肌重构。Objective To investigate the effects of recombinant human brain natriuretic peptide（ rh BNP） on the myocardial remodeling and connexin 43（ Cx43） expression of rats with cardiac failure caused by pressure overload. Methods Forty-five male SD rats were randomly divided into the heart failure group（ group F）,heart failure ＋ rh BNP group（ group B）,and sham operation group（ group SO） with 15 rats in each group. In the group F and group B,we established the rat models of pressure overload-induced heart failure by abdominal aortic constriction,while in the SO group,rats did not receive coarctation of the abdominal aorta. Rats in the group B were subcutaneously injected with rh BNP（ 25 ug/kg/d）,while rats in the group SO and group F were injected intraperitoneally with normal saline for 8 weeks. The left ventricular function,hemodynamics,QT dispersion,left ventricular myocardial pathology,myocardial fibrosis and collagen deposition,and myocardial Cx43 protein expression were detected in each group. Results Compared with the SO group,the left ventricular function,hemodynamics,QT dispersion,left ventricular myocardial histopathology,myocardial fibrosis deposition,myocardial Cx43 protein expression were statistically significant in the group F（ all P〈0. 05）,which showed that heart failure model was established successfully. Compared with group F,the heart function of rats increased,the mean arterial pressure and QT dispersion decreased,the disordered arrangement of myocardial cells reduced,collagen deposition decreased between cardiac myocytes,and the expression of Cx43 increased in the group B（ all P〈0. 05）. Conclusion rh BNP can improve the cardiac function and hemodynamic indexes in rats with heart failure under pressure overload,upregulate the myocardial expression of Cx43 protein,and inhibit myocardial remodeling.

关 键 词：心力衰竭 重组人脑利钠肽 缝隙连接 链接蛋白43 心肌重构 

分 类 号：R541.6[医药卫生—心血管疾病]