A practical approach to generate suitable de novo synthesis RNA template for a flavivirus RNA-dependent RNA polymerase  

作　　者：Wei Shi Peng Gong 

机构地区：[1]Key Laboratory of Special Pathogens and Biosafety,W uhan Institute of Virology,Chinese Academy of Sciences,W uhan 430071.China [2]University of Chinese Academy of Sciences,Beijing l 00049,China

出　　处：《Virologica Sinica》2017年第4期331-334,共4页中国病毒学（英文版）

基　　金：supported by the National Key Basic Research Program of China(2013CB911100 to P.G.);the National Natural Science Foundation of China(31370198 and 31670154);the Open Research Fund Program of Wuhan National Bio-Safety Level 4 Laboratory of Chinese Academy of Sciences(NBL2017009);the“One-Three-Five”Strategic Programs,Wuhan Institute of Virology,Chinese Academy of Sciences(Y605191SA1)

摘　　要：Due to its high transcription efficiency, bacteriophage T7RNA polymerase （T7 RNAP） has long been utilized inbacterial and in vitro systems to generate large quantityof RNA for various purposes （Studier and Moffatt,1986）. However, heterogeneity at the 3＇-end of the RNAtranscript remains a major limitation for in vitro RNApreparation using T7 RNAP. When approaching the endof its DNA template, T7 RNAP tends to add a few extranucleotides not directed by the template strand sequence,resulting in a mixture of RNA products that are equal toor longer than the desired length （Milligan et al., 1987）.

关 键 词：中国病毒学 英文版 

分 类 号：Q78[生物学—分子生物学]