PRL-3抑制剂对子宫内膜异位症大鼠异位病灶的作用  

Effect of PRL-3 inhibitor on the ectopic lesions in rats with endometriosis

作　　者：彭容 詹宏[1] 林俊[1] Peng Rong Zhan Hong Lin Jun(Women＇s Hospital, Zhejiang University school of Medicine, Hangzhou 310006)

机构地区：[1]浙江大学医学院附属妇产科医院,杭州310006

出　　处：《现代妇产科进展》2017年第9期678-681,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:建立子宫内膜异位症动物模型,探索PRL-3抑制剂对SD大鼠异位病灶的影响。方法:选取36只雌性SD大鼠,将自体子宫内膜移植至腹壁造模,免疫组化实验鉴定病灶内膜的上皮及间质组织。内异症大鼠腹腔注射PRL-3抑制剂,按给药浓度分组:对照组(0mg/kg)、低剂量组(0.1mg/kg)、中剂量组(1mg/kg)和高剂量组(10mg/kg),比较处理前后各组病灶情况。结果:SD大鼠自体子宫内膜移植建模存活率为86.1%,存活大鼠的造模成功率为92.5%。SD大鼠各组处理后对比处理前,对照组病灶明显增大(P<0.05),低剂量组和中剂量组病灶大小无明显变化,高剂量组病灶显著缩小(P<0.05)。高剂量组的病灶显著小于对照组(P<0.05)。结论:SD大鼠内异症模型可用于内异症的体内实验研究,是一种操作性强、重复性好且成本低的内异症模型。一定剂量的PRL-3抑制剂可抑制SD大鼠自体异位内膜病灶的生长;PRL-3抑制剂对内异症的靶向治疗有一定的潜在价值。Objective：To set up animal models of endometriosis and explore the effect of PRL-3 inhibitor on endometriotic lesions of SD rats.Methods：36 female SD rats were autotransplanted by endometrium to induce endometriosis.Epithelial and stromal tissues were confirmed by immunohistochemistry in the ectopic lesions.The rats were divided into four groups by intraperitoneal injection of PRL-3 inhibitor,according to different concentrations：control group（0 mg/kg）,low dose group（0.1 mg/kg）,middle dose group（1 mg/kg） and high dose group（10 mg/kg）.The ectopic lesions were analyzed and compared after the treatment of PRL-3 inhibitor.Results：Compare the post-administration with the pre-administration,ectopic lesions of control group were significantly extended（P〈0.05）,while lesions of high-dose group were significantly atrophic（P〈0.05）,and no obvious difference were observed in low-and mid-dose groups.The ectopic lesions of high dose group were significantly lower than that of the control group after administration（P〈0.05）.Conclusion：SD rats model of endometriosis is suitable for the study of endometriosis in vivo,which is operable,reproducible and low cost.A certain concentration of PRL-3 inhibitor can suppress the development of ectopic lesions of SD rats model of endometriosis.There may be the potential value of the PRL-3 inhibitor in the targeted therapy of endometriosis.

关 键 词：子宫内膜异位症 动物模型 PRL-3 PRL-3抑制剂 

分 类 号：R711.71[医药卫生—妇产科学]