盐负荷和醛固酮诱导乳鼠原代心肌细胞肥大的实验研究  

Experimental research on effect of hypertrophy of primary cardiomyocytes in suckling mouse induced by salt loading and aldosterone

作　　者：由淑萍[1] 蒋红[1] 何丽娟[2] 陶宁[2] 王忠[2] 陈清杰 刘涛[2] YOU Shuping JIANG Hong HE Lijuan TAO Ning WANG Zhong CHEN Qingjie LIU Tao(School of Nursing, Xinjiang Medical University, Xingjiang Uygur Autonomous Region, Urumqi 830011, China College of Publie Health, Xinjiang Medical University, Xingjiang Uygur Autonomous Region, Urumqi 830011, China First Affiliated Hospital of Xinjiang Medical University, Xingjiang Uygur Autonomous Region, Urumqi 830054, China)

机构地区：[1]新疆医科大学护理学院,新疆乌鲁木齐830011 [2]新疆医科大学公共卫生学院,新疆乌鲁木齐830011 [3]新疆医科大学第一附属医院,新疆乌鲁木齐830054

出　　处：《中国医药导报》2017年第28期13-16,F0004,共5页China Medical Herald

基　　金：新疆维吾尔自治区自然科学基金资助项目(2015211C020)

摘　　要：目的探讨盐负荷和醛固酮对乳鼠原代心肌细胞肥大的协同作用。方法原代培养乳鼠心肌细胞,随机分为正常对照组、醛固酮10~7 mol/L(Ald)组、等钠浓度140 mmol/L(NS)组、高钠浓度146 mmol/L(HS)组、等钠浓度140 mmol/L+Ald 10~7 mol/L(NS+Ald)组、高钠浓度146 mmol/L+Ald 10~7 mol/L(HS+Ald)组;其中NS+Ald与HS+Ald组先不加Ald,培养48 h后,加入Ald,所有处理组继续培养24 h。免疫荧光法测定心肌细胞横截面积差异,细胞活性实验(CCK-8)检测其活性,流式细胞仪检测细胞凋亡率,荧光定量PCR检测内皮素-1(ET-1)、一氧化氮合酶(eNOS)mRNA表达的差异。结果 NS+Ald组及HS+Ald组心肌细胞横截面积较Ald组、NS组、HS组明显增加(P<0.01);与正常对照组比较,NS+Ald组及HS+Ald组心肌细胞存活率明显下降(P<0.01)、凋亡率显著上升(P<0.01);除NS组外,余组ET-1 mRNA表达升高,eNOS mRNA表达下降,以HS+Ald组最为显著(P<0.01)。结论盐负荷和醛固酮是诱导心肌细胞肥大的重要参与因子,具有协同作用;其中以高盐状态下,醛固酮诱导的心肌细胞肥大最为显著。Objective To investigate the synergistic effects of salt loading and aldosterone on hypertrophy of primary cardiomyocytes in neonatal rats. Methods Neonatal rat cardiomyocytes were cuhuredin the experiment, the cell was divided into normal control group, Aldosterone 10-7 mol/L （Ald） group, normal sodium concentration 140 mmol/L （NS） group, high sodium concentration 146 mmol/L （HS） group, 140 mmol/L＋Ald 10-7 mol/L （NS＋Ald） group, 146 mmol/L＋ Ald 10-7mol/L （HS＋Ald） group. At first Ald was not added into （NS＋Ald） group and （HS＋Ald） group, after 48 h culture, Aid was added, then all treatment groups were continued to culture for 24 h. The difference of myocardial crosssectional area was measured by immunofluorescence. The survival rate was detected by CCK-8. The apoptosis rate was detected by flow cytometry. ET-1 and eNOS mRNA expressions were assayed by qRT-PCR. Results The cross-sectional area of myocardial cells in NS＋Ald group and HS＋Ald group were significantly higher than Aid group, NS group, HS group. Compared with normal control group, the survival rate of cardiomyocytes decreased significantly （P 〈 0.01） and apoptosis rate increased significantly （P 〈 0.01） in NS＋Ald group and HS＋Ald group; except for NS group, ET-1 mRNA expression increased and eNOS mRNA expression decreased in other groups, the effect of HS＋Ald group was the most significant （P 〈 0.01）. Conclusion Salt loading and aldosterone are important participation factors which induced cardiomyocyte hypertrophy, they have synergistic effects. Among them, aldosterone-induced cardiomy-ocyte hypertrophy is the most significant in high salt condition.

关 键 词：盐负荷 醛固酮 心肌细胞 基因表达 凋亡 

分 类 号：R332[医药卫生—人体生理学]