不同工艺芪葵颗粒对糖尿病肾病模型大鼠的保护作用  

Protective effects of different processes of Qikui Particles for model rats with diabetic nephropathy

作　　者：刘靑 朱萱萱[2] 王琼[2] 刘志辉[2] 余江毅[2] 杨晨[2] 符蕊[2] 张迪[2] LIU Qing ZHU Xuanxuan WANG Qiong LIU Zhihui YU Jiangyi YANG Chen FU Rui ZHANG Di(Clinical College of Traditional Chinese Medicine, Gansu University of Traditional Chinese Medicine, Gansu Province, Lanzhou 730000, China Pharmacology Laboratory, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province, Nanjing 210029, China)

机构地区：[1]甘肃中医药大学中医临床学院,甘肃兰州730000 [2]南京中医药大学附属医院药理室,江苏南京210029

出　　处：《中国医药导报》2017年第28期25-30,共6页China Medical Herald

基　　金：江苏省科学技术厅省级科技创新与成果转化(重大科技支撑与自主创新)专项引导资金项目(BE2012777)

摘　　要：目的比较不同工艺芪葵颗粒对糖尿病肾病模型大鼠的保护作用。方法选用健康SD鼠54只,雄性,8周龄。采用高糖高脂饲料+单侧肾脏结扎术+腹腔注射STZ(40 mg/kg)的方法建立糖尿病肾病模型,造模成功后将其分为五组:模型组、厄贝沙坦组、工艺1组(水煮浓缩方)、工艺2组(水煮醇提浓缩方)、工艺3组(水提醇提浓缩合并方),另设空白组。三种不同工艺芪葵颗粒以相同剂量18.67 g/kg分别于造模成功后连续给药5周,观察不同工艺芪葵颗粒对糖尿病肾病大鼠血糖、尿蛋白、肌酐、内生肌酐清除率的影响,并行病理组织学检查。结果与空白组比较,模型组大鼠体重显著降低(P<0.01),进食量、饮水量、尿量明显增加(P<0.05或P<0.01),血糖明显升高(P<0.01)。给药干预后,与模型组比较,各给药组体重不同程度增加,进食量、饮水量、尿量不同程度减少;工艺2组和工艺3组血糖明显降低(P<0.05);厄贝沙坦组和工艺3组血肌酐明显降低(P<0.05或P<0.01);各给药组内生肌酐清除率明显升高(P<0.05或P<0.01);厄贝沙坦组以及工艺2、3组尿蛋白排泄率明显降低(P<0.01或P<0.05)。病理组织学结果显示,模型组大鼠肾脏主要表现为肾小球肥大、肾小管扩张、间质及肾盂炎细胞灶性浸润;与模型组比较,各给药组肾脏病变范围和程度略有减轻,以厄贝沙坦组和工艺2组肾小球和肾小管病变减轻略为明显。结论不同工艺芪葵颗粒对糖尿病肾病的保护作用与降低血糖、肌酐和尿蛋白排泄和减轻肾脏病变有关。以工艺2组与工艺3组较为明显。Objective To compare the protective effects of different processes of Qikui Particles for model rats with diabetic nephropathy. Methods Fifty-four healthy SD rats （male, 8 weeks old） were selected. The model rats with diabetic nephropathy were induced by high sugar/fat diet ＋ unilateral nephritic ligation ＋ Streptozotocin （STZ, 40 mg/kg, ip）. After successful establishment of model rats, they were divided into 5 groups： model group, Irbesartan group, process 1 （water boiled concentrated decoction） group, process 2 （water boiled and alcohol extraction concentration） group, process 3 （the mixed of above two processes） group, and the blank group was set up. Qikui Particles prepared under 3 different processes were orally administered at the same dose （18.67 g/kg ） for 5 consecutive weeks. The effects of differ- ent processes of Qikui Particles on blood sugar, urinary protein, creatinine and endogenous ereatinine clearance rate in rats with diabetic nephropathy were observed, and histopathology was detected. Results Compared with blank group, the body weight in model group was decreased significantly （P 〈 0.01）, the food-intake, water consumption and urine volume were increased （P 〈 0.05 or P 〈 0.01）, the blood sugar was improved significantly （P 〈 0.01）. After drug intervention, compared with model group, the body weight in each drug administration group was increased more or less, the food-intake, water consumption and urine volume food-intake, water consumption and urine volume were decreased more or less; the blood sugar in process 2 group and process 3 group was decreased signifieantly （P 〈 0.05）; the serum creatinine in Irbesartan group and process 3 group was decreased significantly （P 〈 0.05 or P 〈 0.01）; the endogenous creatinine clearance rate in each drug administration group was increased significantly （P 〈 0.05 or P 〈 0.01）; the urinary protein excretion rate in Irbesartan group and process 2, 3 group was decreased significantly （

关 键 词：糖尿病肾病 芪葵颗粒 血糖 肌酐清除率 尿蛋白排泄率 病理组织学 

分 类 号：R285.5[医药卫生—中药学] R587.2[医药卫生—中医学]