针叶小爪螨不同种群内共生菌Wolbachia和Cardinium的检测及系统发育分析  

Detection of Wolbachia and Cardinium Endosymbionts in Different Oligonychus ununguis Populations and Phylogenetic Analysis of Cardinium

作　　者：孙菲[1] 杨春红[1] 李波[2] 郑金柱 周成刚[1] 谢丽霞[1] 尹淑艳[1] Sun Fei Yang Chunhong Li Bo Zheng Jinzhu Zhou Chenggang Xie Lixia Yin Shuyan(College of Plant Protection, Shandong Agricultural University Tai＇an 271018 Forestry Bureau of Taian City Tai＇an 271000 Forest Farm of Mount Culai Tai＇an 271000)

机构地区：[1]山东农业大学植物保护学院,泰安271018 [2]泰安市林业局,泰安271000 [3]泰安市徂徕山林场,泰安271000

出　　处：《林业科学》2017年第8期141-148,共8页Scientia Silvae Sinicae

基　　金：山东省高等学校科技计划项目(J14LE14);国家自然科学基金项目(31401957)

摘　　要：【目的】针叶小爪螨的山东板栗种群(TSBL)和浙江杉木种群(ZJSM)间存在生殖不亲和,而内共生菌Wolbachia和Cardinium能引起宿主的胞质不亲和等生殖异常现象,明确Wolbachia和Cardinium在针叶小爪螨中的分布情况,为下一步研究其在该螨种群分化中的可能作用及其对该螨的生殖调控作用奠定基础。【方法】提取单头雌成螨的总DNA,使用特异性引物通过PCR扩增Wolbachia的wsp基因和Cardinium的16S rRNA基因的部分片段,将电泳检测为阳性的PCR产物纯化、测序,以确定所得产物是否为目的基因产物,并进行系统发育分析。【结果】针叶小爪螨的山东板栗种群(TSBL)和浙江杉木种群(ZJSM)均无Wolbachia和Cardinium感染。所检测的12个针叶小爪螨种群中,Wolbachia和Cardinium的感染率分别是0和8.3%。Cardinium仅在河北麻栎种群中检测到,但种群内感染率较高,为66.7%。感染针叶小爪螨的Cardinium与感染二斑叶螨的Cardinium的亲缘关系较近。【结论】我国针叶小爪螨中Wolbachia的感染率可能极低,该螨山东板栗种群(TSBL)和浙江杉木种群(ZJSM)间的生殖不亲合与Wolbachia和Cardinium无关。【Objective 】 There exists reproductive incompatibility between the Castanea mollissima population from Shandong Province （TSBL） and the Cunninghamia lanceolata population from Zhejiang Province （ZJSM） of Oligonychus ununguis.However,Wolbachia or Cardinium,as the insect ＇s endosymbiont,has the ability to alter the reproductive capabilities of its host.This study aims to analyze the distribution of Wolbachia and Cardinium in different O.ununguis populations,and to reveal the effects of endosymbionts on O.ununguis reproductive incompatibility.【Method】 Primers were designed according to the sequences of the gene encoding a surface protein of Wolbachia （wsp） and the Cardinium＇s16S rRNA gene,and PCR was performed using the genomic DNA extracted from a single adult female O.ununguis.Purified PCR fragments were sequenced and blasted against the databases.Phylogenetic tree was constructed based on these sequences.【Result】 Neither the C.mollissima population from Shandong Province （TSBL） nor the C.lanceolata population from Zhejiang Province （ZJSM） was infected with Wolbachia.In all the detected 12 populations of O.ununguis,the infection rates of Wolbachia and Cardinium were 0 and 8.3%,respectively.Cardinium was detected only in the Quercus acutissima population from Hebei Province,but the intrapopulation infection rate was high with 66.7%.The Cardinium infecting O.ununguis was closer to that from Teranychus urticae in phylogenetic relationship.【Conclusion 】The infection rate of Wolbachia might be very low in O.ununguis in China.The reproductive incompatibility between the C.mollissima population from Shandong Province （TSBL） and the C.lanceolata population from Zhejiang Province （ZJSM） of O.ununguis was independent of Wolbachia and Cardinium.

关 键 词：针叶小爪螨 种群分化 WOLBACHIA CARDINIUM 系统发育 

分 类 号：S718.83[农业科学—林学]