慢性低压低氧对大鼠肺组织HIF-1ɑ、COX1蛋白及氧化应激反应的影响  

Effects of chronic hypobaric hypoxia on HIF-1α amd COX1 proteins as well as oxidative stress in lung tissue of rats

作　　者：马生龙 李胜[2] 李晓娜 米国霞 靳国恩[4] 李生花 MA Sheng-long LI Sheng LI Xiao-na MI Guo-xia JIN Guo-en LI Sheng-hua(Dept. of Cardiology, Qinghai Cardiovascular and Cerebrovascular Disease Hospital, Xining 810012 Dept. of Plague Prevention and Control Qinghai Institute for Endemic Disease Prevention and Control, Xinirtg 811602 Dept. of Basic Medical Science, Qinghai University Medical College, Xining 810001 Research Center for High Altitude Medicine, Qinghai University Medical College, Xining 810001, China)

机构地区：[1]青海省心脑血管病专科医院心内科,青海西宁810012 [2]青海省地方病预防控制所鼠疫预防控制科,青海西宁811602 [3]青海大学医学院基础医学部,青海西宁810001 [4]青海大学医学院高原医学研究中心,青海西宁810001

出　　处：《基础医学与临床》2017年第10期1355-1358,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(81550040);青海大学医学院青年基金(2016-KY-7)

摘　　要：目的探讨慢性低压低氧环境对大鼠肺组织细胞色素C氧化酶1(COX1)蛋白及氧化应激反应的影响。方法将大鼠随机分为常氧组(1 500 m)和低氧组(4 300 m),低氧组大鼠暴露低氧30 d后采集标本。用Western blot检测肺组织COX1蛋白的表达及ELISA检测肺组织和血清HIF-1ɑ、血浆ROS、肺组织SOD和CAT酶。用生理信号采集系统测定肺动脉压(PAP)。结果在低氧组的大鼠血清和肺组织内HIF-1α蛋白显著高于常氧组(P<0.01);ROS含量显著低于常氧组(P<0.01);COX1蛋白在低氧组的大鼠肺组织中表达显著下降(P<0.01);血清总抗氧化能力升高(P<0.01)。结论高海拔对机体的影响可能是直接性的损伤,而并非仅仅是氧化应激反应所致。Objective To investigate the effects of chronic hypobaric hypoxia on COX1 protein and oxidative stress in lung tissue. Methods Rats were randomly divided into normoxia group（ 1 500 m） and hypoxia group（ 4 300 m）. The rats in hypoxia group were sampled after exposure to hypoxia for 30 days. The COX1 protein in lung tissue of rats was determined by Western blot method; HIF-1αlevel in lung tissue and serum,ROS in plasma and SOD,CAT enzyme in lung tissue were determined by ELISA method; PAP of rats were determined by physiological signal acquisition system. Results HIF-1 protein express in serum and lung tissue of rats in hypoxia group was significantly higher than that of the normoxia group（ P〈0. 01）,the content of ROS was significantly lower than that of the normoxia group（ P 0. 01）,the expression of COX1 protein in lung tissues of hypoxia group was significantly decreased（ P 0. 01）,serum total antioxidant capacity was elevated in hypoxia group（ P〈0. 01）.Conclusions The effects of chronic hypobaric hypoxia on lung tissue may be caused by direct injury,not only by oxidative stress.

关 键 词：高海拔 低压低氧 氧化应激 肺组织 COX1 

分 类 号：R594.3[医药卫生—内科学]