他米巴罗汀减轻大鼠脑缺血再灌注损伤  

Tamibarotene alleviates cerebral ischemia reperfusion injury in rats

作　　者：安瑞娣 田晓翠 杨梅 李明航 徐露 董志 AN Rui-di TIAN Xiao-cui YANG Mei LI Ming-hang XU Lu DONG Zhi(Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China)

机构地区：[1]重庆医科大学药学院重庆市生物化学与分子药理学重点实验室,重庆400016

出　　处：《基础医学与临床》2017年第10期1424-1428,共5页Basic and Clinical Medicine

基　　金：重庆市教委资助项目(KJ1600235)

摘　　要：目的探讨他米巴罗汀(Am80)对大鼠脑缺血再灌注损伤(CIR)的作用。方法将大鼠随机分为:假手术组(sham)、模型组(I/R)和他米巴罗汀干预组(Am80,灌胃给予Am80 6 mg/kg)。采用线栓法建立大脑中动脉栓塞(MCAO)模型。术后24 h断头取脑,在处死前采用双盲法进行神经行为学评分;TTC染色测定脑梗死体积;Western blot和RT-q PCR法分别检测RARα、Bcl-2和Bax蛋白及mRNA的表达。结果 Am80可显著改善MCAO大鼠神经功能缺损,有效地降低脑梗死体积(P<0.01),上调RARα和Bcl-2表达(P<0.01),降低Bax的表达(P<0.01)。结论他米巴罗汀对脑缺血再灌注损伤大鼠有保护作用,其作用可能与抗凋亡有关。Objective To investigate the effect of tamibarotene（ Am80） on cerebral ischemia reperfusion injury.Methods Rats were randomly divided into sham operation group（ Sham）,model group（ I/R） and tamibarotene intervention group（ Am80,intragastric administration of Am80 6 mg/kg）. The middle cerebral artery occlusion（ MCAO） was established by suture method. At 24 h after reperfusion,the brain was removed. And the neurological behavioral score was assessed by double blind method. The cerebral infarction volume was measured by TTC staining.The proteins of RARα,Bcl-2,Bax and mRNA were examined by Western blot and RT-q PCR,respectively. Results Am80 significantly improved the neurological deficits of MCAO rats and effectively reduced the infarct volume. And upregulated the level of RARα and Bcl-2,decreased the expression of Bax. Conclusions Am80 has a protective effect on cerebral ischemia-reperfusion in rats,and its effect may be related to anti-apoptosis.

关 键 词：他米巴罗汀 脑缺血再灌注损伤 凋亡 

分 类 号：R965[医药卫生—药理学]