不同免疫程序对猪蓝耳病疫苗免疫效果的影响  

The evaluation of different immunization schedules for PRRS vaccine effect

作　　者：孙雪[1] 赵一霏 李帅[1] 郭士琪[1] 董烨[1] 张飞[1] 沈国顺[1] SUN Xue ZHAO Yi-fei LI Shuai GUO Shi-qi DONG Ye ZHANG Fei SHEN Guo shun(College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161 ,China)

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110161

出　　处：《中国兽医学报》2017年第10期1847-1851,共5页Chinese Journal of Veterinary Science

基　　金：辽宁省农业公关及产业化项目(2014202004)

摘　　要：为研究不同免疫程序对猪蓝耳病疫苗免疫效果的影响,本试验选取42头未免健康断奶仔猪,随机分为4组。A组对照组,B组免疫弱毒苗组,C组免疫灭活苗组,D组同时免疫弱毒苗和灭活苗组。分别在免疫前和免疫后第14,28,42,56,70天前腔静脉采血,采用ELISA方法检测特异性抗体,同时检测淋巴细胞转化率以及IFN-α、IFN-γ、TNF-α等细胞因子水平。结果显示:免疫后,B组抗体水平显著高于D组(P<0.05)、A组(P<0.01);在免疫后第42天,D组淋巴细胞转化率显著高于B组、C组(P<0.01),显著高于A组(P<0.01),免疫后第56天,B组淋巴细胞转化率高于C、D组(P<0.05);B组细胞因子水平在免疫后第14天高于其他3组,加强免疫后细胞因子水平均有所下降,在免疫后第70天时升高。结果表明,免疫蓝耳病弱毒苗组免疫效果优于免疫灭活苗组和两种疫苗同时免疫组。In order to investigate the effect of different immunization schedules of PRRS vaccine. Forty healthy and weaned piglets were randomly divided into 4 groups （A,B,C,D）. Group A was control group, group B with attenuated immunity, group C with inactivated immunity, group D with attenuated and inactivated immunity. Blood samples were taken from cranial vena cava on the 14th,28th,42th, 56th, 70th day after immunity, the levels of specific antibodies were detected by ELISA, and lymphocyte transformation rate and the levels of cytokines IFN-α, IFN-γ, TNF-α were detected by ELISA. The results showed;the level of antibodies in group B was higher than group D （P〈0.05） and group A （P〈0.01）. On the 42th day after immune,the lymphocyte transforma- tion rate in group D was significantly higher than group A,B and C （P〈0.01）;the lymphocyte transformation rate of group B was higher than group C and D （P〈0.05） after the 56th day,The levels of cytokine in group B was higher than the other groups on the 14th day,after enhanceing immune,the levels decreased,then increased on the 70th day. The results indicated that the effect of attenuated vaccine immunity is higher than other two kinds of immunization.

关 键 词：蓝耳病 疫苗 体液免疫 细胞免疫 

分 类 号：S852.65[农业科学—基础兽医学]