猪脾转移因子增强几种重要禽疫苗免疫效果的测试研究  

Test study on reinforcing immune efficacy of several important avian vaccine by porcine spleen transfer factor

作　　者：徐磊[1,2] 陈月香 刘道泉 邱艳红 胡美华[2] 谭礼宁 谢兆文 刘友霖 刘俊斌[1] 黄瑜[5] 朱国强[1] XU Lei CHEN Yue-xiang LIU Dao-quan QIU Yan-hong HU Mei-hua TAN Li-ning XIE Zhao-wen LIU You lin LIU J un bin HUANG Yu ZHU Guo-qiang(College of Veter- inary Medicine, Yangzhou University, Yan g zhou , J iangsu 225009, China Fuj ian Agricultural Vocational Technical College ,Fuzhou 350119,China Institute of Animal Husbandry and Vet- erinary Medicine , Fuj ian Academy of Agricultural Sciences , Fuzhou 350013, China Fuzhou Animal Disease Prevention and Control Center,Fuzhou 350002, China S. Present （Fuzhou） Bio- tech Company Limited ,Fuzhou 350500 ,China)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]福建农业职业技术学院,福建福州350119 [3]福州市动物疫病预防控制中心,福建福州350002 [4]派生特(福州)生物科技有限公司,福建福州350500 [5]福建省农业科学院畜牧兽医研究所,福建福州350013

出　　处：《中国兽医学报》2017年第10期1944-1950,共7页Chinese Journal of Veterinary Science

基　　金：2015年度“福建省高校杰出青年科研人才培育计划”(闽教科[2015]54号);2015年福建省中青年教师教育科研项目计划(JA15760);福建省畜禽疫病防控技术重大研发平台项目(2014N2003);2016年度福建省科技计划项目(2016S0002);2017年度福建省科技计划项目(2017N0009);2015年福州市科技计划项目(2015-G-71);2016年福州市科技计划项目(2016-G-64);2016年福建农业职业技术学院科研项目(2016JS005)

摘　　要：为研究猪脾转移因子(TF)对禽疫苗的免疫增强作用,采用血凝抑制(HI)试验和琼脂凝胶扩散沉淀(AGP)试验方法测试了TF分别与鸡新城疫(ND)病毒活疫苗、禽流感(AI)病毒灭活疫苗、鸡传染性支气管炎(IB)病毒灭活疫苗、鸡传染性法氏囊病(IBD)病毒活疫苗联合接种SPF鸡后抗体效价(log2x)动态变化(共4个试验组),同时运用ELISA法评估了所采用免疫程序(上述前3种疫苗与TF同时接种后第7天,进行上述第4种疫苗与TF的联合接种)对SPF鸡外周血IFN-γ、IL-2、IL-4和IL-10的影响(试验组5),于接种上述第4种疫苗后的7,14,21,28,35,42d检测细胞因子,均设立对照组(上述禽疫苗单独接种)和空白组(非疫苗接种)。结果显示,接种后7,14,21,28,35,42d,试验组的ND血凝抑制(HI)、AI HI、IB HI和IBD琼脂扩散沉淀(AGP)抗体效价均显著高于对照组(P<0.05),分别提高了1.1~1.4,1.0~1.3,0.7~1.4,0.9~1.6;试验组的IL-2、IFN-γ和IL-4浓度分别于接种后7,14,21d显著高于对照组和空白组(P<0.05),各组的IL-10浓度差异不显著。研究结果表明,TF可增强NDV活疫苗、AIV灭活疫苗、IBV灭活疫苗和IBDV活疫苗的免疫效果。To study on reinforcing immune efficacy of avian vaccine by porcine spleen transfer factor （TF）, antibody titer （log., x） of SPF chickens inoculated with Newcastle Disease Virus （NDV） live vaccine and TF, Avian Influenza Virus （AIV） inactivated vaccine and TF, Infectious Bronchitis Virus （IBV） inactivated vaccine and TF,Infectious Bursal Disease Virus （IBDV） live vaccine and TF respectively （4 test groups） was detected by Hemagglutination Inhibition （HI） test and Agar Gel Diffusion Precipitation （AGP） test,and also IFN-γ,IL 2,IL-4,IL-10 of SPF chickens followed theimmunization program （inoculation with the fourth vaccines mentioned above and TF on 7 day post inoculation with the above 3 vaccines and TF） on 7,14,21,28,35 and 42 day post inoculation with the fourth vaccines mentioned above were detected by ELISA （test group 5）. Meanwhile,the control group （the above avian vaccine singly） and blank group （non vaccination） were established accordingly. The results showed that on 7,14,21,28,35 and 42 day post inoculation, the titers of ND HI,AI HI,IB HI and IBD AGP in the 4 test groups were significantly higher than those in the control groups （P〈0.05） ,respectively,1.1-1.4,1.0-1.3,0.7-1.4 and 0.9-1.6 were increased. The concentrations of IL-2, IFN-γ and IL-4 in the test group 5 were significantly higher than in the control group and the blank group （P〈0.05） on 7,14 and 21 day post inoculation, respectively. There was no significant difference in IL-10 concentration between groups. These findings indica- ted that TF could enhance the immune efficacy of NDV live vaccine,AIV inactivated vaccine, IBV inactivated vaccine,IBDV live vaccine.

关 键 词：猪脾转移因子 禽疫苗 HI抗体效价 AGP抗体效价 细胞因子 

分 类 号：S852.65[农业科学—基础兽医学]