母体铅暴露对仔鼠肝脏MMP-2和MMP-9 mRNA表达的影响  

Effect of maternal lead exposure on the MMP-2 and MMP-9 mRNA expression in the liver of mouse offsprings

作　　者：李丽[1] 崔灿[1] 李宁[1] LI Li CUI Can LINing(College of Food Science and Technology, Henan Agriculture University, Zhengzhou 450002, Chin)

机构地区：[1]河南农业大学食品科学技术学院,河南郑州450002

出　　处：《中国兽医学报》2017年第10期1964-1968,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31201878;U1204804);中国博士后基金资助项目(2015M572109);河南省博士后基金资助项目(2014049)

摘　　要：为探讨母体铅暴露对仔鼠肝脏MMP-2和MMP-9 mRNA表达的影响,采用自由饮水模式建立铅暴露动物模型,将40只雌性小鼠自妊娠第1天开始经饮水染铅(1.0,5.0,10.0g/L,对照组饮蒸馏水)至仔鼠出生后21d,随机分为低、中、高剂量染毒组和对照组,仔鼠21日龄,分别测其血液和肝脏中铅的含量,然后取其肝脏组织,通过实时荧光定量PCR(Real-time PCR)技术检测各组仔鼠肝脏中MMP-2和MMP-9mRNA的表达情况。结果显示,21d后,铅暴露组仔鼠血液和肝脏中铅水平均明显高于对照组(P<0.05);与对照组相比,低、中和高剂量铅暴露组仔鼠肝脏内MMP-2mRNA的表达量明显增高,且差异具有统计学意义(P<0.05)。中、高剂量铅暴露组仔鼠肝脏组织中MMP-9mRNA的表达明显高于对照组(P<0.05),但低剂量铅暴露组仔鼠肝脏组织中MMP-9mRNA的表达与对照组相比差异不显著(P>0.05)。结果表明,母体铅暴露可能通过改变仔鼠肝脏中MMP-2 mRNA的表达进而造成肝脏损伤。母体铅暴露使铅在仔鼠体内蓄积,铅暴露可能通过增强仔鼠肝脏中MMP-9mRNA的表达进而造成肝脏损伤,从而引起组织发生病变。To explore the effect of maternal lead exposure on the MMP-2 mRNA and MMP-9 expression in the liver of mouse offsprings. The animal model of lead exposure was established by adopting a way of free drinking water. Forty female mice were divided into low, medium and high dosages of infected groups 1.0,5.0 and 10.0 g/L,respectively and the control group. The test las- ted from 1 d pregnancy to 21 d after giving birth to mouse offsprings. The control group drank dis- tilled water. At 21-day-old offsprings,the contents of lead in blood and liver was detected,using re- al-time fluorescent quantitative PCR methods to detect liver expression of MMP 2 mRNA and MMP-gmRNA. The lead levels in blood and liver of lead exposure groups were significantly higher than those of control group （P〈 0.05）;comparing with the control group,MMP-2 mRNA expression in low, medium and high lead exposure groups were higher （P〈0.05）. The expression of MMP-9 mRNA in liver of middle and high lead exposure group was higher than the control group （P〈0.05） ,but there was no difference between low lead exposure group and the control group（P〉0.05）. Maternal lead exposure may induce lead accumulation in the liver of offspring, resulting in a changed of MMP-2mRNA and MMP-gmRNA expression and liver damage.

关 键 词：铅 肝脏 MMP-2 MMP 9mRNA 实时荧光定量PCR 小鼠 

分 类 号：R995[医药卫生—毒理学]