电针预处理对大鼠脑缺血再灌注损伤后脑梗死体积及血清TNF-α、IL-10含量的影响  

Effects of electroacupuncture preconditioning on cerebral infarct volume and contents of TNF-α,IL-10 in serum of rats after cerebral ischemia-reperfusion injury

作　　者：叶涛[1] 朱路文[2] 唐强[2] 李宏玉[1] 吴孝军[1] 姜云飞[2] YE Tao ZHU Luwen TANG Qiang LI Hongyu WU Xiaojun JIA NG Yunfei(Heilongjiang University of CM, Harbin 150040, China the Second Hospital Affiliated to Heilongjiang University of CM, Harbin 150001)

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]黑龙江中医药大学附属第二医院,哈尔滨150001

出　　处：《中国针灸》2017年第10期1093-1098,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金:81503666;黑龙江省自然科学基金:QC 2015103;哈尔滨市科技创新人才专项基金(青年后备人):2014 RFQGJ 150;黑龙江中医药大学领军人才计划项目:2012 RCL 02;黑龙江中医药大学研究生创新科研项目:yjscx 2016037

摘　　要：目的:观察电针预处理对大鼠脑缺血再灌注损伤后脑梗死体积及血清肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)含量的影响,探索预防脑卒中的方法。方法:采用随机数字表将36只大鼠随机分为假手术组、模型组、电针预处理组共3组,每组12只。这3组内又分为再灌注12 h和再灌注24 h 2个亚组,每个亚组6只大鼠。电针预处理组大鼠造模前给予连续2周的电针干预。模型组和电针预处理组采用改良Longa线栓法制备大鼠脑缺血再灌注模型。再灌注12 h、24 h,采用改良行为学评分量表评估各组大鼠神经功能缺损程度,TTC法观测脑梗死体积,酶联免疫分析(ELISA)法检测血清TNF-α、IL-10含量。结果:再灌注12 h、24 h,与模型组比较,电针预处理组神经功能缺损评分均明显较低(均P<0.05),脑梗死体积均明显较低(均P<0.05)。再灌注12 h、24 h,与假手术组比较,模型组TNF-α、IL-10含量均较高(均P<0.05);再灌注12 h,与模型组比较,电针预处理组血清TNF-a含量较低(P<0.05),而血清IL-10含量较高(P<0.05);再灌注24 h,与模型组比较,电针预处理组血清TNF-a、IL-10含量均较低(P<0.05)。结论:电针预处理可以改善大鼠脑缺血再灌注损伤后神经功能缺损,缩小脑梗死体积,发挥脑保护作用,其机制可能与电针能调节脑缺血再灌注急性期外周循环血中促炎因子TNF-a与抗炎因子IL-10间动态平衡,对抗炎性反应加剧有关。Objective To explore the effect of electroacupuncture（EA） preconditioning on cerebral infarct volume and the contents of TNF-α,IL-10 in serum of rats with cerebral ischemia-reperfusion injury.Methods Thirty-six rats were randomly divided into a sham operation group,a model group and an EA preconditioning group,12 rats in each group,which were further divided into 12 h and 24 h after reperfusion subgroups,6 rats in each one.EA was used before model establishment for 2 weeks in the EA preconditioning group.The model of cerebral ischemia-reperfusion injury in rats was established with modified Longa suture method.12 h and 24 h after reperfusion,the degree of neurological deficit was assessed by the modified behavioral scoring scale;the cerebral infarct volume was measured by TTC method and the contents of TNF-α,IL-10 in serum were detected by ELISA method.Results Compared with the model group,the neurological severity scores in the EA preconditioning group significantly reduced 12 h and 24 h after reperfusion（both P〈0.05）,the cerebral infarct volume in the EA preconditioning group significantly reduced 12 h and 24 h after reperfusion（both P〈0.05）.Compared with the sham operation group,the serum TNF-α,IL-10 contents in the model group increased 12 h and 24 h after reperfusion（both P〈0.05）.Compared with the model group,the serum TNF-α content reduced,while the serum IL-10 content increased in the EA preconditioning group 12 h after reperfusion（both P〈0.05）.Compared with the model group,the serum TNF-α,IL-10 contents reduced in the EA preconditioning group 24 h after reperfusion（both P〈0.05）.Conclusion EA preconditioning can improve neurological deficit,reduce cerebral infarct volume after cerebral ischemia-reperfusioninjury in rats.The mechanism may be related to the regulation of EA on the dynamic balance between pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 in peripheral blood of cerebral ischemia-reperfusion injury in acute phase,thus alleviate acute ce

关 键 词：脑缺血再灌注 电针预处理 脑梗死体积 肿瘤坏死因子-α 白细胞介素-10 炎性反应 

分 类 号：R245.9[医药卫生—针灸推拿学]