靶细胞萃取联用UPLC/Q-TOF-MS方法分析川芎中的药效成分  

Analysis of Pharmacodynamic Components in Chuanxiong Rhizoma by Target Cell Sorption Combined with UPLC/Q-TOF-MS

作　　者：谢佳宾 王媛[1] 田梦[1] 李亚梅 武媛媛[1] 王玉明[1] 李遇伯[1] 张艳军[2] 

机构地区：[1]天津中医药大学中药学院,天津300193 [2]天津中医药大学天津市现代中药重点实验室,天津300193

出　　处：《中国现代中药》2017年第9期1245-1248,共4页Modern Chinese Medicine

基　　金：国家科技支撑计划课题(2011BAI07B08)

摘　　要：目的:建立靶细胞萃取联用UPLC/Q-TOF-MS技术分析川芎中药效成分的方法。方法:选用人脐静脉内皮细胞(HUVEC)作为靶细胞,萃取川芎中的药效成分。在细胞对数生长期加入川芎供试品溶液,孵育2 h收集细胞并用液氮迅速冷冻,反复冻融使细胞破碎,离心得到萃取药物后的细胞样品。采用UPLC/Q-TOF-MS对其进行检测,分析川芎化学成分中与细胞结合的物质,即川芎中与细胞结合进一步发挥药效的可能有效成分。结果:从川芎中筛选到2个可能的药效成分,分别为阿魏酸和藁本内酯。结论:本研究建立的方法可以通过化合物与细胞中靶点(受体、通道、酶等)之间的特异性结合作用,筛选出川芎中药效活性成分,从而为药物活性成分的筛选以及药物机制的研究提供思路,同时推动了中药安全性的快速发展。Objective： To establish a method by using target cell and UPLC/Q-TOF-MS for extraction the pharmacodynamic components in Chuanxiong Rhizoma. Methods： The human umbilical vein endothelial cells （HUVEC） were used as target cells, and the freeze-dried powder of Chuanxiong Rhizoma in logarithmic growth period was added. After 2 h incubation, the cells were collected and frozen rapidly with liquid nitrogen, the cells were broken by repeated freezing and thawing, and cen- trifuged to obtain a sample of cells after extraction. While compounds in the extract of cell samples were determined by UPLC/Q- TOF-MS, the effect of possible effective components that entered the cell of Chuanxiong Rhizoma was analyzed. Results： After HUVEC extraction two active ingredients ferulic acid and ligustilide were obtained. At the same time, the method was estab- lished for the determination of the two components. Conclusion ： The analysis method of the target cell extraction and UPLC/Q- TOF-MS can be applied for screening out the active ingredients in Chuanxiong Rhizoma by specific binding effect between the compound and the target （receptor, channel, enzyme, etc. ） in the cell, so as to provide a new method for screening the ac- tive ingredients and drug mechanism study.

关 键 词：川芎 靶细胞萃取 藁本内酯 阿魏酸 UPLC/Q—TOF—MS 

分 类 号：R282.71[医药卫生—中药学]