花生种质资源耐低温表型鉴定方法研究  

Research of Identification Method on Low Temperature Resistance of Peanut Germplasm Resources Phenotype

作　　者：刘海龙[1,2] 陈小姝 杨富军[1] 白冬梅[3] 孙晓苹[1] 吕永超[1] 任小平[2] 姜慧芳[2] 高华援[1] LIU Hai-long CHEN Xiao-shu YANG Fu-jun BAI Dong-mei SUN Xiao-ping LU Yong-chao REN Xiao-ping JIANG Hui-fanga GAO Hua-yuan(Peanut Research Institute, J ilin Academy of Agricultural Sciences, Gongzhuling 136100, China Economic Plant Research Institute, Shanxi Academy of Agricultural Sciences, Fenyang 032200, China Key Lab. of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China)

机构地区：[1]吉林省农业科学院花生研究所,吉林公主岭136100 [2]中国农业科学院油料作物研究所,湖北武汉430062 [3]山西省农业科学院经济作物研究所,山西汾阳032200

出　　处：《花生学报》2017年第3期20-25,共6页Journal of Peanut Science

基　　金：农业部油料作物生物学与遗传育种重点实验室开放课题基金(2014001);农业部948项目(2015-Z55);国家花生产业技术体系(CARS-14);吉林省农业科学院创新工程-花生创新团队(CXGC2017TD013);吉林省农业科技创新工程杰出青年项目(CXGC2017JQ005);中国农业科技东北创新中心/博士后研究项目

摘　　要：本研究目的是构建既可鉴定花生种质资源耐低温属性,又可进行耐低温性数量性状分析的表型鉴定方法。本方法构建公式相对发芽率=x_i/y_i×100%,花生耐低温性属性分级标准鉴定花生耐低温属性,及利用相对发芽率数据进行耐低温数量性状分析。利用此方法对94份花生种质资源进行鉴定,鉴定出相对发芽率≥85%耐低温型(R)6个,约占检测资源的6.38%,分别为山花8(A1)、豫花1(A38)、开农30(A10)、白沙1016(A37)、油4(A32)和泉花646(A42),从94份花生资源中选取20份材料进行田间耐低温验证,其结果与本研究构建的方法结果一致,证明花生种质资源耐低温表型方法可以鉴定花生种质资源耐低温属性;利用此方法鉴定出徐花13×中花6重组自交系群体(RIL)各家系及亲本,分析耐低温性主基因+多基因遗传模型,符合模型G-1,主基因的遗传率为91.21%,多基因的遗传率为8.34%,证明此方法可应用在耐低温性数量性状分析上。This study was to construct an identification method which can simultaneously identify low temperature tolerance characteristic of peanut germplasm resources and analyze low temperature resistance quantitative traits. The formula adopted as RGRi （relative germination rate） =xi/yi×100%. 94 peanut germplasm resources were identified with RGB as： 6 germplasm resources, Shan- hua8 （A1）, Yuhual （A38）, Kainong30 （A10）, Baisha1016 （A37）, You4 （A32） and Quanhua646 （A42）, were low temperature resistance based on the RGB≥85%. 20 germplasm resources selected from 94 were verified in field, which showed that the results were consistent with the result throughthe identification method. RIL and parents were identified with this method. Through analysis of low temperature resistant major gene and polygene genetic model, the results showed that the peanut low temperature resistance characteristic was accordant with model G-l, and the major gene heritability was 91.21%, polygene heritability was 8.34%. This method could be applied to analyze the low tem- perature resistance quantitative characteristic of peanut.

关 键 词：花生 耐低温 表型 相对发芽率 

分 类 号：S565.201[农业科学—作物学]