亚甲基四氢叶酸还原酶C677T基因多态性与2型糖尿病关系的病例对照研究  

Relationship between Methylenetetrahydrofolate Reductase Gene Polymorphism and Type 2 Diabetes Mellitus

作　　者：商广芸[1] 王宏宇[1] 刘金波[1] 袁平[1] 周迎燕 李丽红[1] Shang Guangyun Wang Hongyu Liu Jinbo et al(Department of Vascular Medicine, Peking University Shougang Hospital, Beifing 100144, China)

机构地区：[1]北京大学首钢医院血管医学科,100144

出　　处：《医学研究杂志》2017年第9期72-75,共4页Journal of Medical Research

基　　金：北京市卫生局首都卫生发展科研专项基金资助项目(首发2011-4026-02);北京大学首钢医院院内基金资助项目(SGYY201406);2015年北京市石景山区科技计划项目;首钢总公司科研项目(2013科管20-1);北京大学首钢医院院内2014年度医院临床重点项目建设课题(2014-院-临床-02)

摘　　要：目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性与2型糖尿病(T2DM)的关系。方法选取226例T2DM患者(病例组)和194例健康对照个体(对照组),检测两组同型半胱氨酸水平,采用PCR-芯片杂交技术检测亚甲基四氢叶酸还原酶C677T多态位点基因型,计算等位基因频率并统计分析。结果 2组MTHFR基因C677T位点基因型和等位基因频率比较,差异均无统计学意义(P>0.05),MTHFR基因TT型同型半胱氨酸水平显著高于CC型及CT型。结论 MTHFR基因C677T多态性影响同型半胱氨酸水平,但与T2DM的发病无明显的相关性。Objective To examine the relationship between methylenetetrahydrofolate reductase （MTHFR） gene C677T polymor- phism and type 2 diabetes mellitus （T2DM）. Methods MTHFR gene C677T polymorphie sites were assayed in 226 T2DM and 194 healthy subjects by PCR - DNA microarray. Homosysteine levels were measured. And their genotype and allele frequency were statistically analyzed. Results There was no statistical significance in allele frequency and genotype of MTHFR gene C677T between the patients and healthy subjects（ P 〉 0.05）. The homosysteine levels were obviously higher in TT genotype. Conclusion MTHFR gene C677T polymor- phism is not a risk factor for patients with type 2 diabetes,but can effect homosysteine levels.

关 键 词：2型糖尿病 亚甲基四氢叶酸还原酶 基因多态性 

分 类 号：R446.1[医药卫生—诊断学]