草木樨中华根瘤菌CHW10B溶磷特性及其对南方红豆杉的促生作用  

Phosphate-solubilizing Characteristics and Growth-promoting Effect of Sinorhizobium meliloti CHW10B on Taxus chinensis var. mairei

作　　者：李云玲[1,2] 侯沁文 刘瑞祥[1,2] 任嘉红 张易[1] LI Yun-ling HOU Qin-wen LIU Rui-xiang REN Jia-hong ZHANG Yi(Department of Biological Science and Technology, Changzhi University, Changzhi 046011, Shanxi, China Ecological and Environmental Research Institute of Taihangshan Mountains, Changzhi 046011, Shanxi, China)

机构地区：[1]长治学院生物科学与技术系,山西长治046011 [2]太行山生态与环境研究所,山西长治046011

出　　处：《林业科学研究》2017年第5期751-758,共8页Forest Research

基　　金：国家自然科学基金项目(31100471);山西省重点学科建设经费资助

摘　　要：[目的]探讨南方红豆杉根际微生物草木樨中华根瘤菌(Sinorhizobium meliloti)CHW10B的溶磷特性及促生作用。[方法]利用液体发酵试验比较不同时间、碳源、氮源、碳氮比、培养温度等条件下CHW10B菌株的溶磷量,采用温室盆栽法研究该菌株对南方红豆杉生长的影响,并分析该菌株产嗜铁素、精氨酸脱羧酶、ACC脱氨酶及有机酸等成分的能力。[结果]该菌株分别在培养时间4 d、磷酸钙5.00 g·L^(-1)、初始p H值7.0、装液量1/2、NaCl为0.0 g·L^(-1)、温度30℃、碳源为葡萄糖、氮源为硫酸铵、碳氮比100∶1时溶磷能力最强。将该菌株接种于1年生南方红豆杉实生苗360 d后,苗木的地径、苗高、生物量比对照分别增长了19.53%、20.14%、25.39%。该菌株能够产嗜铁素、精氨酸脱羧酶、ACC脱氨酶(0.922 U·mg^(-1))和IAA(8.908 mg·m L^(-1)),并可分泌大量有机酸—葡萄糖酸(5 704.92μg·L^(-1))。[结论]草木樨中华根瘤菌CHW10B溶磷能力强,对南方红豆杉促生作用显著,适用于多种不同酸碱性土壤,可高效应用于南方红豆杉的人工栽培,具有被开发为生物肥料的潜力。[ Objective] A phosphate-solubilizing bacterium, Sinorhizobium meliloti CHW10B, was selected from Taxus chinensis var. mairei. Its phosphate-solubilizing characteristics and growth-promoting effect on T. chinensis vat. mairei were studied. [ Method] The effects of culture time, Ca3 （PO4） 2 concentration, carbon sources, nitro- gen sources, initial pH, liquid filling volume, salt ions and other environment factors on phosphate solubilizing ability of strain CHW10B were studied by liquid fermentation experiment. Pot experiment was conducted to evaluate the role of strain CHW10B in promoting the growth of T. chinensis var. mairei. Some growth-promoting mechanism such as producing ACC deaminase, siderophore, indoleacetic acid, arginine decarboxylase and organic acid were deter- mined. [ Result] The results showed that CHW10B strain displayed the highest phosphate-dissolving capacity when the cultivation period was 4 days, the amount of calcium phosphate was 5.00 g· L-1, the initial pH reached 7.0, the volume of liquid was 1/2, the NaC1 concentration was 0.0 g · L-l and the temperature was 30℃. The phos- phate solubilizing capacity of the strain got the highest when using glucose as carbon source and ammonium sulfate as nitrogen source. Moreover, the ratio of carbon to nitrogen （ 100：1 ） is optimum for strain CHW10B. After being inoculated with strain CHWIOB, the ground diameter, seedling height and biomass of 1-year-old T. chinensis var. mairei seedlings increased by 19.53%, 20.14% and 25.39% in 360 days＇ post-inoculation, respectively. The strain could produce siderophore, arginine decarboxylase, ACC deaminase （0.922 U · mg-1 ） and IAA （8. 908 mg·mL-1 ）, and secrete a large amount of gluconic acid （5 704.92 μg· L-1 ）. [ Conclusion] S. meliloti CHW10B has strong phosphorus-solubilizing capability, and plays a significant promoting role in the growth of T. chinensis var. mairei. The strain can be applied to a variety of soils of different acidity-alkalinity. The result would be an impor

关 键 词：草木樨中华根瘤菌 南方红豆杉 溶磷 促生作用 

分 类 号：S718.8[农业科学—林学]