早竹丛枝病的调查及病原菌的分子鉴定  

Disease Severity Assessing and Molecular Identification of Pathogen Associated with Witches' Broom Disease of Phyllostachys praecox

作　　者：耿显胜[1] 张威[1] 仲建平 张守科[1] 舒金平[1] GENG Xian-sheng ZHANG Wei ZHONG Jian-ping ZHANG Shou-ke SHU Jin-ping(Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, Zhejiang, China Forestry Technology Extension Station of Deqing County, Zhejiang Province, Deqing 313200, Zhejiang, China)

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江杭州311400 [2]浙江省德清县林业技术推广站,浙江德清313200

出　　处：《林业科学研究》2017年第5期805-811,共7页Forest Research

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2014QA006)

摘　　要：[目的]对早竹丛枝病进行调查及病原菌分子鉴定,为早竹丛枝病的病害诊断和防治提供科学依据。[方法]采用单株水平和单枝盘水平的2种病害分级标准对早竹丛枝病进行调查。使用植原体16S rDNA和真菌rDNA-ITS序列的特异性PCR引物,对早竹丛枝病的病原菌进行分子鉴定。[结果]调查的6块样地早竹丛枝病的平均发病率为18.59%,平均病情指数为6.67。感病的早竹DNA样品能够扩增出真菌的rDNA-ITS序列,而不能够扩增出植原体的16S rDNA序列;扩增出的序列与报道的竹针孢座囊菌的序列同源性达到99.00%,与其它真菌的序列同源性最高仅为94.00%。[结论]浙江省德清县早竹丛枝病的病原菌为竹针孢座囊菌。[ Objective] In this study, the witches＇ broom disease of Phyllostachys praecox was assessed, and the pathogen of the disease was identified through molecular biology techniques. [ Method ] The disease severity was as- sessed by grading witches＇ broom disease at individual level and branch level. The pathogen of witches＇ broom dis- ease of Ph. praecox was identified by specific PCR primers of phytoplasmal 16S rDNA and fungal rDNA-ITS se- quences. [ Result] Disease assessment data showed that the average incidence of the 6 plots was 18.59%, and the average disease index was 6.67. PCR results indicated that all the samples infected were able to amplify rDNA-ITS sequence of fungus, and could not amplify 16S rDNA of phytoplasma. Comparative BLAST analysis determined that the amplicons shared 99.00% similarity with sequences from Aciculosporium take, and the highest similarity with other fungi was only 94.00%. [ Conclusion]A. take was the causal agent of witches＇ broom disease of Ph. praecox of Deqing County, Zhejiang Province.

关 键 词：早竹 竹子丛枝病 竹针孢座囊菌 分子鉴定 病情指数 

分 类 号：S795.9[农业科学—林木遗传育种]