三元(碱-表面活性剂-聚合物)复合驱油藏采出水微生物群落特征  

Characterization of microbial community in produced water from a petroleum reservoir subjected to alkali-surfactant-polymer(ASP) flooding

作　　者：郝钦钦 史荣久[1] 郝金生[3] 赵劲毅[3] 李国桥[3] 赵峰[1] 韩斯琴[1] 张颖[1] HAO Qin-qin SHI Rong-jiu HAO Jin-sheng ZHAO Jin-yi LI Guo-qiao ZHAO Feng HAN Si-qin ZHANG Ying(Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China University of Chinese Academy of Sciences, Beijing 100049, China No. 2 Oil Production Plant of Daqing Oilfield Co. Ltd., Daqing 163414, Heilongfiang , China）.)

机构地区：[1]中国科学院沈阳应用生态研究所,中国科学院污染生态与环境工程重点实验室,沈阳110016 [2]中国科学院大学,北京100049 [3]大庆油田有限责任公司第二采油厂,黑龙江大庆163414

出　　处：《应用生态学报》2017年第10期3393-3402,共10页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(31570121);中国科学院污染生态与环境工程重点实验室开放课题资助~~

摘　　要：与水驱技术相比,向油藏中注入碱、表面活性剂和聚合物(简称三元复合驱,ASP)能大幅提高石油采收率,但这些驱油剂对油藏中微生物多样性与群落结构的影响尚亟待阐明,这对油田水质管理与腐蚀控制均具有的重要意义.本研究采用高通量测序技术解析了大庆油田ASP油藏4口油井采出水中的微生物多样性与群落结构.结果表明:ASP油藏采出水的p H高达9.65.采出水中微生物Shannon多样性指数为2.00~3.56,采出井间菌群多样性存在差异.在门、纲、属分类水平上,变形菌门(85.5%~98.3%)、γ-变形菌纲(83.7%~97.8%)、栖碱菌属(51.8%~82.5%)是绝对优势菌群.共检测到12个属的潜在硫化氢产生菌,以硫磺单胞菌属丰度最高(0.4%~7.4%).与已发表的水驱油藏研究结果相比,三元复合驱油藏采出水微生物群落组成独特,呈嗜/耐碱趋势,其多样性偏低,群落结构更单一.Injection of alkali,surfactant and polymer（ ASP） into oil reservoir can substantially increase oil recovery compared with water-flooding strategy. However,the effects of these agents on the microbial diversity and community structure,which is important for water management and corrosion control in oil industry,are hitherto poorly understood. Here,we disclosed the microbial diversity and community structure in the produced water collected from four producing wells of an ASP-flooded oilfield at Daqing, China, using high-throughput sequencing technique. Results showed that the average p H in produced water was as high as 9.65. The microbial diversity varied from well to well,and the Shannon diversity index was between 2. 00 to 3. 56. The Proteobacteria（ 85.5%-98. 3%）,γ-proteobacteria（ 83. 7%-97. 8%）,and alkaliphilic Nitrincola（ 51. 8%-82.5%） were the most dominant phylogenetic taxa at the phylum,class,and genus levels,respectively. A total of 12 potentially sulfide-producing genera were detected,and the most abundant taxon was Sulfurospirillum（ 0.4%-7.4%）. The microbial community of ASP-flooded petroleum reservoir was distinct,showing an alkaliphilic or alkalitolerant potential; a reduced diversity and more simple structure were observed compared with those of the water-flooded petroleum reservoirs that were previously reported.

关 键 词：三元复合驱 油藏 微生物群落结构 高通量测序 硫酸盐还原菌 采出水 硫化氢 

分 类 号：Q93[生物学—微生物学] TE357.46[石油与天然气工程—油气田开发工程]