基于碳点标记的(1-3)-β-D葡聚糖的荧光检测方法  

A High Selective and Specific Fluorescent Test Method for The Detection of (1,3)-β-D-Glucan

作　　者：葛宇新 张洋[2] 田振华[2] 于源华[2] 郭瑞[2] 张昊[2] 

机构地区：[1]长春理工大学校医院,长春130022 [2]长春理工大学生命科学技术学院,长春130022

出　　处：《长春理工大学学报（自然科学版）》2017年第4期133-137,共5页Journal of Changchun University of Science and Technology(Natural Science Edition)

基　　金：吉林省科技厅资助项目(20140309013YY)

摘　　要：血清中(1,3)-β-D葡聚糖(βG)的含量已经成为临床上检测侵袭性真菌感染的指标,利用原核表达并纯化获得能够与βG特异性结合的美洲鲎属G因子α亚基片段a(Gαa)蛋白,将Gαa蛋白与碳点偶联获得生物荧光传感器"碳点/Gαa蛋白",建立基于碳点标记的βG荧光检测方法。经测定该传感器的最佳的激发波长为360nm,其发射波长为470nm,且荧光强度与βG浓度呈正线性相关,该检测方法线性范围是9.375~150pg/m L,最低检测限为9.375pg/m L,批间、批内精密度均不小于5%,准确度(回收率)在97%~102%之间,该方法灵敏、快速、成本低,具有广阔的应用前景。The concentration of（1,3）-beta-D glucan（βG） in human serum is a clinical testing index of invasive fungal infection,a beta-glucan binding protein of horseshoe crab（limulus polyphemus） factor G-subunit α fragment-a（Gαa） was expressed in Escherichia coli BL21,Coupling of amination retouching carbon dots with G protein and establishing a＂carbon dots/Gαa protein＂fluorescent sensor for βG detection. The sensor excitation wavelength was 360 nm,emission wavelength was 460 nm.The fluorescent and the concentration of βG were a positive linear correlation.,the detection range was 9.375~150pg/m L,the limit of detection was 9.375 pg/m L. The CV values within batch and between batches were all less than 5%,accuracy（recovery） were between 97%~102%. The novel fluorescent sensor for the detection of βG was low cost,rapid,high selective,sensitivity and specificity.

关 键 词：(1 3)-β-D葡聚糖 美洲鲎属G因子α亚基片段a特异性蛋白 碳量子点 真菌感染 

分 类 号：O613.71[理学—无机化学]