利用探针跳跃式离子电导显微镜研究活体血小板膜结构及其膜微粒的形成  

Real-time investigation of dynamic morphology of live platelets and generation of platelet microparticles using hopping probe ion conductance microscopy

作　　者：刘晓 罗玉福[2] 张彦军[3,4] 

机构地区：[1]国家纳米技术与工程研究院,天津300457 [2]天津市永久医院,天津300450 [3]天津医科大学总医院神经外科 [4]天津市神经病学研究所,天津300052

出　　处：《生物医学工程学杂志》2017年第5期767-771,共5页Journal of Biomedical Engineering

基　　金：天津市科技计划(14ZCZDSY00020);天津市滨海新区塘沽科技兴区项目(2012XQ15-11);国家自然科学基金青年科学基金项目(31300828)

摘　　要：血小板在激活剂作用下会快速活化并释放具有高促凝活性的血小板膜微粒(PMPs),进而诱发出凝血功能障碍,但PMPs的形成机制有待明确。探针跳跃式离子电导显微镜(HPICM)具有在生理环境下对活体细胞进行非接触式实时高分辨率成像的技术优势。本文运用HPICM技术实时监测血小板在胞内钙离子诱导剂A23187和细胞松弛素D作用下活化及PMPs形成的过程,证明了胞内钙离子浓度和细胞骨架蛋白在血小板活化及PMPs形成中发挥了重要作用;并发现与扁平型血小板相比,突起型血小板对A23187和细胞松弛素D更为敏感,这对利用HPICM技术研究血小板活化与出凝血功能调节之间的关系具有指导意义。Platelets are rapidly activated by activators and produce a large number of platelet microparticles （PMPs） with high coagulation activity, resulting in coagulation dysfunction. However, the generation mechanism of PMPs is still not clear. Hopping probe ion conductance microscopy （HPICM） has special technical advantages in non-contact, real-time, high-resolution imaging of living cells under physiological conditions. Using HPlCM, this study monitored the processes of platelet activation and generation of PMPs in real time in the presence of calcium ionophore A23187 and cytochalasin D （CD）, respectively. The results proved that the intraceUular calcium concentration and the cytoskeletal proteins played important roles in the platelet activation and the generation of PMPs. Compared with the low density spread shape platelets （LDSS）, the high density bubble shape platelets （HDBS） were more sensitive to the calcium ionophore A23187 and cytochalasin D. This research has a guiding significance for the further study on the relationship between platelet activation and coagulation function using HPICM.

关 键 词：探针跳跃式离子电导显微镜 血小板活化 血小板膜微粒 

分 类 号：R446.1[医药卫生—诊断学]