用于浮游菌研究的微环境实验箱系统的研制  

Development of a microenvironment test chamber for airborne microbe research

作　　者：詹宁波[1,2] 陈锋[1] 杜耀华[1] 程智[1] 李辰宇[1] 吴金龙[1] 吴太虎[1] 

机构地区：[1]军事医学科学院卫生装备研究所,天津300161 [2]解放军第三〇二医院医学工程科,北京100039

出　　处：《生物医学工程学杂志》2017年第5期778-783,共6页Journal of Biomedical Engineering

摘　　要：浮游菌是衡量环境洁净度的一项重要指标,然而洁净作业环境与受控实验环境的特殊性往往导致浮游菌的研究比较受限。本文设计并实现了一种微环境实验箱系统,便于在常规实验条件下进行浮游菌研究。Fluent数值模拟表明,浮游菌在实验箱内中上部具备较好的分布状态,并呈现出自下而上的浓度增长分布现象。根据模拟结果,在实验箱内选择不同空间位置的5个测量点进行粒子计数浓度验证实验。实验结果表明金黄色葡萄球菌发菌5 min后各测量点平均粒子浓度均达到了10~7个/m^3数量级,各测量点粒子分布较为一致,且上部浓度略高于中部浓度,中部浓度略高于底部浓度。验证实验结果与数值模拟结果相符,证明该系统能够较好地用于浮游菌研究。One of the most important environmental cleanliness indicators is airborne microbe. However, the particularity of clean operating environment and controlled experimental environment often leads to the limitation of the airborne microbe research. This paper designed and implemented a microenvironment test chamber for airborne microbe research in normal test conditions. Numerical simulation by Fluent showed that airborne microbes were evenly dispersed in the upper part of test chamber, and had a bottom-up concentration growth distribution. According to the simulation results, the verification experiment was carried out by selecting 5 sampling points in different space positions in the test chamber. Experimental results showed that average particle concentrations of all sampling points reached 107 counts/m^3 after 5 minutes＇ distributing of Staphylococcus aureus, and all sampling points showed the accordant mapping of concentration distribution. The concentration of airborne microbe in the upper chamber was slightly higher than that in the middle chamber, and that was also slightly higher than that in the bottom chamber. It is consistent with the results of numerical simulation, and it proves that the system can be well used for airborne microbe research.

关 键 词：浮游菌 微环境 实验箱 

分 类 号：R318[医药卫生—生物医学工程]