异氟烷预处理增强大鼠海马内M2型小胶质细胞活化减轻电磁脉冲所致神经损伤  

Isoflurane preconditioning enhances the activation of M2 microglia in hippocampus of rats to reduce the nerve injury caused by electromagnetic pulse

作　　者：张霞婧[1,2] 田丽颖 王婧[2] 喻倩 王晋[3] 邵勇平[1] 孙绪德[2] 

机构地区：[1]西安市第四医院麻醉科,西安710005 [2]第四军医大学唐都医院麻醉科,西安710038 [3]第四军医大学放射医学教研室,西安710032

出　　处：《神经解剖学杂志》2017年第5期526-530,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(3157040065)

摘　　要：目的:观察异氟烷预处理是否通过增强大鼠海马内M2型小胶质细胞活化,减轻炎症反应改善电磁脉冲所致神经元损伤。方法:24只SD大鼠随机分为4组,分别为对照组(CON组)、异氟烷预处理组(IP组)和电磁脉冲照射组(EMP组)和异氟烷预处理加电磁脉冲照射组(IP+EMP组)。各组于照射后24 h进行尼氏染色;采用Western Blot和免疫荧光组织化学染色方法检测M2型小胶质细胞标志物Arg-1的表达;并采用ELISA方法测定海马组织中TNF-α和IL-1β的含量。结果:与CON组相比,EMP组海马齿状回颗粒细胞的尼氏体明显减少,而与EMP组相比,IP+EMP组海马齿状回颗粒细胞的尼氏体明显增多增大。Western Blot结果显示:与CON组相比,EMP组海马内Arg-1蛋白的表达明显减少(P<0.05),与EMP组相比,IP+EMP组Arg-1蛋白的表达明显增加(P<0.05)。免疫荧光染色结果显示:与CON组相比,EMP组海马内Arg-1的表达减弱;而与EMP组相比,IP+EMP组Arg-1的表达明显增强。EMP组海马组织中炎症因子TNF-α和IL-1β的含量较CON组增加(P<0.05),IP+EMP组脑组织中炎症因子TNF-α和IL-1β的含量较EMP组减少(P<0.05)。结论:异氟烷预处理可通过增加M2型小胶质细胞,减少脑组织中炎症因子含量,减轻EMP所致神经元损伤。Objective： To observe whether isoflurane preconditioning improves electromagnetic pulse induced neuronal damage by enhancing the activation of M2 microglia in hippocampus of rats and reducing the inflammatory response. Metheds： 24SO rats were randomly divided into four groups： control group （CON group）, isoflurane preconditioning group （IP group）, electromagnetic pulse group （EMP group） and isoflurane preconditioning plus electromagnetic pulse group （IP ＋ EMP group）. Nissl staining was performed in each group 24 hours after irradiation; The expression of Arg-1 was detected by Western Blot and immunofluorescence staining, and the concentration of TNF-α and IL-1β in hippocam- pus were determined by enzyme-linked immunosorbent assay. Results： Compared with the CON group, the Nissl bodies of hippocampal granule cells decreased in the EMP group; compared with EMP group, the Nissl bodies of hippocampal granule cells increased in the IP ＋ EMP group. Western Blot showed that the expression of Arg-1 protein in＂ EMP group was significantly decreased （P 〈 0.05 ） comparing with CON group, and compared with EMP group, the expression of Arg-1 protein in IP ＋ EMP group was significantly increased （ P 〈 0.05 ）. Immunofluorescence staining shows that Compared with the CON group, the the expression of Arg-1 protein in the EMP group was decreased, while the expression of Arg-1 protein in IP ＋ EMP group was significantly increased compared with the EMP group. The concentration of TNF- ot and IL-113 in hippocampus were increased in the EMP group （ P 〈 0.05 ） comparing with CON group, and compared with the EMP group, the concentration of TNF-α and IL-1β decreased significantly in the IP ＋ EMP group （ P 〈 0. 05 ）. Conclusion： Isoflurane preconditioning can reduce proinflammatory factor in the brain by increasing M2 microglia activa- tion, and alleviate EMP neuronal damage.

关 键 词：异氟烷预处理 电磁脉冲 小胶质细胞 神经元 大鼠 

分 类 号：R614[医药卫生—麻醉学]