转化生长因子β1对脂变HepG2．2．15细胞HBV复制及抗原合成的影响  

Effect of transforming growth factor-β1 on HBV replication and antigen synthesis in HepG2.2.15 cellswith steatosis

作　　者：王艳[1] 赵龙风[1] 王荣荣[1] 智陞雯[1] 

机构地区：[1]山西医科大学第一医院感染病科,太原030001

出　　处：《中华肝脏病杂志》2017年第10期732-737,共6页Chinese Journal of Hepatology

基　　金：山西省高等学校科技创新项目（20141103）

摘　　要：目的探讨转化生长因子β1（TGFβ1）对脂变HepG2．2．15细胞HBV复制及蛋白表达的影响，以及Hep32／HepG2．2．15脂变过程中TGFβ1与细胞因子信号抑制物-3（SOCS-3）mRNA、固醇调节元件结合蛋白1c（SREBP—1c）mRNA的关系。方法细胞分为HepG2／HepG2．2．15细胞对照组（C1／C2组）和脂变组（F1／F2组），并在这两组细胞体系内加入5ng／m1TGFp1干预，形成TGFD1作用组（T1／T2）和脂变＋TGFβ1组（TF1／TF2），采用时间分辨荧光分析仪检测HBsAg、HBeAg，实时荧光定量PCR法检测HBVDNA、SOCS-3mRNA及SREBP-1cmRNA。数据采用单因素方差分析以及析因分析进行统计。结果TGFβ1能显著降低C2组中HBeAg水平（P=0．034），及显著降低F2组中HBsAg（P〈0．001）以及HBeAg（P=0．004）水平。脂变与TGFβ1对下调HBsAg有交互作用。此外，TGFβ1能显著降低C1、F1、C2及F2组中SOCS-3mRNA表达（P〈0．05），同时显著升高C1、F1、C2及F2组中SREBP-1cmRNA的表达（P〈0．05），脂变与TGFβ1对抑制SOCS-3mRNA以及促进SREBP-1cmRNA的表达有交互作用。结论TGFβ1不影响HepG2．2．15细胞中HBV复制，可抑制HBsAg和HBeAg的表达；TGFβ1可抑制SOCS-3mRNA的表达，及促进SREBP—1ctuRNA的表达。Objective To investigate the effect of transforming growth factor-β1 （TGF-β1） on HBV replication and protein expression in HepG2,2.15 cells with steatosis, as well as the association of TGF-β1 with suppressor of cytokine signaling-3 （SOCS-3） mRNA and sterol regulatory element-binding protein-1 c （SREBP- 1 c） mRNA during the steatosis of HepG2.2.15 cells. Methods The cells were divided into HepG2/HepG2.2.15 cell control groups （C1/C2 groups） and HepG2/HepG2.2.15 cell steatosis groups （F1/F2 groups）. 5 ng/ml TGF-β1 was added to the two eell systems for intervention to establish TGF-β1 intervention groups （T1/T2 groups） and steatosis＋TGF-β1 intervention groups （TF1/TF2 groups）. A time-resolved fluorescence analyzer was used to measure HBsAg and HBeAg, and quantitative real-time PCR was used to measure HBV DNA, SOCS-3 mRNA, and SREBP-1 mRNA. A one-way analysis of variance and a factorial analysis were used for the statistical analysis of data. Results TGF-β1 significantly reduced the level of HBeAg in C2 group （P = 0.034） and the levels of HBsAg （P 〈 0.001） and HBeAg （P = 0.004） in F2 group. There was an interaction between steatosis and TGF-β1 in inhibiting HBsAg. In addition, TGF-β1 significantly reduced the mRNA expression of SOCS-3 in C1, F1, C2, and F2 groups （P 〈 0.05） and significantly increased the mRNA expression of SREBP-lc in C1, F1, C2, and F2 groups （P 〈 0.05）, suggesting that there was an interaction between steatosis and TGF-β1 in downregulating themRNA expression of SOCS-3 and upregulating the mRNA expression of SREBP-1c. Conclusion TGF-β 1 does not affect HBV duplication in HepG2.2.15 cells and can inb_ibit the expression of HBsAg and HBeAg. TGF-β1 can downregulate the mRNA expression of SOCS-3 and upregulate the mRNA expression of SREBP- 1 c.

关 键 词：肝炎  乙型 转化生长因子Β1 非酒精性脂肪肝 细胞因子信号抑制物-3 固醇调节元件结合蛋白-1C 

分 类 号：R512.62[医药卫生—内科学]