西瓜枯萎病生防细菌的筛选、鉴定及其抑菌作用  

Screening,identification and inhibitory effect of antagonistic bacterium on Fusarium oxysporum f. sp. nevium

作　　者：李萍[1] 刘冬[1,2] 朱秀蕾[1] 姜晓斌[1] 毕璋友[1] LI Ping LIU Dong ZHU Xiulei JIANG Xiaobin BI Zhangyou(Department of Horticulture and Landscape, Anqing Vocational and Technical College, Anqing 246003 School of Plant Protection, Anhui Agricultural University, Hefei 230036, China)

机构地区：[1]安庆职业技术学院园林园艺系,安徽安庆246003 [2]安徽农业大学植物保护学院,安徽合肥230036

出　　处：《仲恺农业工程学院学报》2017年第3期24-28,共5页Journal of Zhongkai University of Agriculture and Engineering

基　　金：安徽省高校自然科学研究项目(KJ2015A368;KJ2013B133);安徽省高校优秀青年人才支持计划重点项目(gxyq ZD2016516)资助

摘　　要：采用平板对峙法从连作西瓜(Citrullus lanatus)的根际土壤中筛选获得1株对西瓜枯萎病菌(Fusarium oxysporum f.sp.niveum)具有较强拮抗作用的细菌BTF5菌株,并对其进行了形态观察、生理生化测定和16S r DNA序列分析鉴定.结果表明:该BTF5菌株对西瓜枯萎病菌的抑制率达63.09%,与Gen Bank中越南伯克霍尔德氏菌(Burkholderia vietnamiensis)具有很高的遗传相似性,同源性为99%.因此将细菌BTF5鉴定为越南伯克霍尔德氏菌.经人工培养基上连续8次传代培养,该细菌对西瓜枯萎病菌的抑制效果稳定.An antagonistic bacterial strain BTF5 against Fusarium oxysporum f.sp.niveum was isolated from rhizosphere soil collected from continual watermelon mono-cropping by dual-culture,and identified according to morphology,biochemical assay and homology analysis of 16S rDNA sequence.The results showed that inhibition rate of the strain BTF5 against F.oxysporum f.sp.niveum was up to 63.09% in the antagonistic experiment.Compared with sequences listed in the Gen Bank database,strain BTF5 had the highest genetic similarity with Burkholderia vietnamiensis and homology is 99%.Less change in the inhibition effect of antagonizing against growth of F.oxysporum f.sp.niveum was observed during eight generations of transfers on artificial media,indicating its potential biocontrol efficacy and application prospect.

关 键 词：西瓜枯萎病菌(Fusarium OXYSPORUM f.sp.niveum) 拮抗细菌 筛选 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]