甲基对硫磷抗性菌的筛选及特性研究  

作　　者：王琳琳 姜彬慧[2] 

机构地区：[1]中机国能炼化工程有限公司,天津300350 [2]东北大学资源与土木工程学院,辽宁沈阳110819

出　　处：《环境保护与循环经济》2017年第5期18-21,27,共5页environmental protection and circular economy

基　　金：国家自然科学基金项目(51278090)

摘　　要：有机磷农药在控制农作物害虫和病虫方面占据重要的地位,是世界上生产和使用最多的农药品种。有机磷农药的毒性及不规范使用,是造成土壤农药污染的主要因素,直接或间接地危害人类的健康,开展污染土壤的修复技术研究意义重大。从受农药长期污染的土壤中筛选出长势较好的3株野生种菌,分别为B_1,B_2和B_3,通过开展3种菌对甲基对硫磷(ParathionMethyl,PM)的降解实验、耐受性实验和PM浓度对降解率影响的实验研究,确定3种菌在12 h时对PM的最大降解率分别为73.04%,78.05%和85.51%,PM对B_2和B_3的最大抑制浓度为600mg/L和800mg/L。The organophosphorus pesticide occupies an important position in control of pests and diseases, which are the most production and application of pesticide varieties in the world. However, due to the toxicity and the nonstan- dard use of organophosphorus pesticide, which was the main factor of soil pesticide contamination, and harm human health directly or indirectly. Therefore, developing contaminated soil bioremediation technology research is of great significance. Three preponderant pure strains were screened and gotten from the long-term pesticides pollution soil lixivium, noted for the bacteria B~, B2, and B3 respectively, through the strain＇s degradation process curve experi- ment, tolerability experiment and the effect substrate concentration on degradation rate experiment, it was obtained that the three bacteria reached the highest degradation rate in 12 h, which was respectively 73.04%, 78.05% and 85.51%, the biggest tolerant concentration of bacteria B2 and B3 was respectively 600 mg/L and 800 mg/L.

关 键 词：甲基对硫磷 分离筛选 生化和降解特性 

分 类 号：Q93[生物学—微生物学]