跑台运动对高脂饮食喂养的肥胖小鼠比目鱼肌脂质沉积和血管内皮生长因子B表达的影响  

Effects of Treadmill Running on Intramyocellular Lipid Deposition and VEGFB Expression in Soleus Muscle of High-fat Diet Mice

作　　者：张靓[1] 马谨[1] 高海宁[2] 李灵杰[1] 

机构地区：[1]北京师范大学体育与运动学院,北京100875 [2]沈阳体育学院运动人体科学学院

出　　处：《中国运动医学杂志》2017年第9期773-778,共6页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金(31471137;81273096)

摘　　要：目的:观察跑台运动对高脂饮食诱导的肥胖小鼠骨骼肌脂质沉积和血管内皮生长因子B(VEGFB)及其受体表达的影响。方法:32只5周龄健康雄性C57BL/6小鼠,随机分成对照组(n=8)和高脂喂养组(n=24),分别进行普通饲料和高脂饲料喂养,8周后筛选肥胖小鼠16只,随机分为高脂安静组和高脂运动组,每组各8只。高脂运动组跑台训练方案为:跑速25 m/min,每次60 min,每周5次,持续8周,对照组和高脂安静组不进行运动。小鼠处死前测定体重,小鼠比目鱼肌油红O染色观察脂质沉积,免疫组化法观察比目鱼肌血管内皮生长因子受体-1(VEGFR1)和辅受体Neuropilin-1(NRP1)的表达水平,Real-time PCR方法测定比目鱼肌VEGFB和受体VEGFR1、NRP1的mRNA表达,以及VEGFB上游调节因子PGC-1αmRNA表达。结果:与对照组相比,高脂饮食喂养小鼠体重显著增加(P<0.01),比目鱼肌中脂质沉积增加,VEGFB、VEGFR1、NRP1和PGC-1αmRNA表达无显著变化。与高脂安静组小鼠相比,高脂运动组小鼠体重显著降低(P<0.01),比目鱼肌中脂质沉积显著减少,VEGFR1 mRNA表达显著上升(P<0.05),但VEGFB、NRP1和PGC-1αmRNA表达无显著变化。结论:跑台运动对肥胖小鼠比目鱼肌VEG-FB的表达无显著作用,但显著上调其受体VEGFR1的表达,提示运动减轻骨骼肌脂质沉积的作用可能与运动增强比目鱼肌VEGFB/VEGFR1作用相关。Objective To observe the effect of treadmill running on skeletal muscle lipid deposition,and the expression of vascular endothelial growth factor-B（VEGFB） and its receptor in soleus muscles of high-fat diet mice.Methods Thirty-two 5-week-old C57BL/6 male mice were randomly divided into a control group （n=8） and a high-fat diet group（n=24）,and fed with normal and high-fat diet respectively.Eight weeks later,16 obesity mice were selected from the latter group and randomly divided into a sedentary group（n=8）and a treadmill running group（n=8）.The running group underwent treadmill running at 25 m/min for an hour every day,five days a week for 8 weeks,while the other two groups did not do any exercises.The weight was measured before the mice were killed.The soleus lipid deposition level was determined using oil red O staining,The expression of VEGFR1 and Neuropilin-1（NRP1）was determined using the immunohistochemical staining,while the mRNA expression of VEGFB,VEGFR1,NRP1 and peroxisome proliferator-activated receptor-γcoactivator-1α（PGC-1α）was detected using the real-time PCR.Results Compared with the control group,a significant increase was observed in the weight and the soleus lipid deposition of the high-fat diet group,while there were no significant differences in the expression of VEGFB,NRP1,VEGFR1 and PGC-1α.mRNA between the two groups.Compared with the sedentary obese mice,significant decrease in the body weight and soleus lipid deposition,significant increase in VEGFR1 mRNA expression,but no significant changes in the expression of VEGFB,NRP1 and PGC-1α mRNA were observed in the treadmill-running obese mice.Conclusion The treadmill running has no effect on the VEGFR1 expression in soleus of obese mice,but significantly up-regulates the expression of its receptors.It indicates that the treadmill running can lower the intramyocellular lipid deposition as it can strengthen the VEGFB/VEGFR1 in soleus muscles.

关 键 词：跑台运动 骨骼肌脂质沉积 VEGFB 肥胖 比目鱼肌 

分 类 号：R87[医药卫生—运动医学]