兴奋剂19-去甲雄酮和19-去甲本胆烷醇酮的检测研究  

Study for the Doping Control of 19-Norandrosterone and 19-Noretiocholanolone with Isotope Ratio Mass Spectrometry

作　　者：王静竹[1] 杨瑞[1,2] 刘欣[1] 

机构地区：[1]国家体育总局反兴奋剂中心,北京100029 [2]北京体育大学,北京100084

出　　处：《中国运动医学杂志》2017年第9期793-796,共4页Chinese Journal of Sports Medicine

基　　金：国家体育总局重点研究领域攻关课题(2013B003)

摘　　要：目的:19-去甲雄酮(19-Norandrosterone,19NA)和19-去甲本胆烷醇酮(19-Noretiocholanolone,19NE)是竞技体育中禁用的内源性类固醇兴奋剂,可由诺龙代谢而来。本研究的目的是采用同位素比质谱方法对尿中19NA和19NE进行检测规律研究。方法:征集2名男性志愿者。口服20 mg诺龙,并于服药前后收集尿样。对尿样中19NA和19NE的浓度及同位素比进行测定。结果:服药后尿样中19NA和19NE的浓度迅速上升,其同位素比降低至接近受试品的同位素比值。结论:摄入诺龙不影响尿中孕烷二醇、雄酮、本胆烷醇酮的同位素比值。尿样在服药后27小时内提示尿样为阳性。Objective The 19-Norandrosterone （19NA） and 19-Noretiocholanolone （19NE） are endogenous steroids abused as dope.The aim of this study was to investigate the detection of 19 NA and19NE using isotope ratio mass spectrometry.Methods Two male volunteers were recruited.Each volunteer was orally administered a single dose of 20 mg of nandrolone,and their urine samples were collected after administration.The analyses of urinary concentrations and isotope ratios of 19 NA and 19 NE were performed.Results Concentrations of 19 NA and 19 NE increased rapidly after administration,and their isotope ratios decreased to the value of the drug ingested.Conclusion The intake of nandrolone has no effect on the isotope ratios of pregnanediol,androsterone and etiocholanolone.The urine samples were positive within 27 hours after administration.

关 键 词：19-去甲雄酮 19-去甲本胆烷醇酮 同位素比质谱 兴奋剂检测 

分 类 号：R872[医药卫生—运动医学]