小鼠动脉粥样硬化模型中miR-23a与血清炎症因子相关性的研究  

Correlation between miR-23a and serum inflammatory factors in mouse model of atherosclerosis

作　　者：孙婷婷[1] 王珺晓 徐扬[1] 仉珊珊 刘亚坤 罗善顺[1] 

机构地区：[1]哈尔滨医科大学附属第一医院干部病房,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2017年第4期296-299,303,共5页Journal of Harbin Medical University

基　　金：国家自然科学基金资助项目(81270366)

摘　　要：目的探讨不同周龄小鼠动脉粥样硬化(atherosclerosis,AS)模型中miR-23a与白介素-6(IL-6)、单核细胞趋化蛋白-1(MCP-1)和肿瘤坏死因子-α(TNF-α)等血清炎症因子的相关性。方法选取12周龄、14周龄、16周龄、18周龄ApoE基因敲除小鼠(ApoE-/-小鼠),各6例,分别采集各组小鼠血清进行实时荧光定量PCR(RT-PCR)检测miR-23a的表达情况,用酶联免疫吸附法(ELISA)检测不同周龄小鼠血清IL-6、MCP-1和TNF-α炎症因子的表达水平。结果随着ApoE-/-小鼠周龄的增加,小鼠血清中miR-23a表达呈逐渐降低趋势,而小鼠血清中的IL-6、MCP-1、TNF-α的表达明显升高。各组间比较差异均有统计学意义(P<0.05)。结论在ApoE-/-小鼠AS形成过程中,随着miR-23a表达水平下降,负向控制IL-6、MCP-1和TNF-α在内的多种炎症因子的表达,增加主动脉组织内的炎症反应水平,加速AS的发生发展。Objective To investigate the correlation between miR-23a and interleukin-6 （IL- 6）, monocyte chemoattractant protein-1 （MCP-1） and tumor necrosis factor-α （TNF-α） and other inflammatory factors in atherosclerotic （AS） models of different age mice. Methods ApoE knockout mice （ApoE-/- mice） at 12 weeks old, 14 weeks old, 16 weeks old and 18 weeks old were selected in 6 cases, the serum of each group was collected and the expression of miR-23a was detected by real-time quantitative PCR （RT-PCR） , and the levels of IL-6, MCP- 1 and TNF-a in the serum of different age mice were detected by enzyme-linked immunosorbent assay （ELISA）. Results With the increase of the age of ApoE-/- mice, the expression of miR-23a in mice serum gradually decreased, while the expression of IL-6, MCP-1 and TNF-α in serum was increased with a statistical significance （P 〈 0. 05）. Conclusion In the process of AS formation of ApoE-/- mice, with the decrease of miR-23a expression level, negative con- trol of a variety of inflammatory factors, including IL-6, MCP-1 and TNF-α, increase the level of inflammatory reaction within the aorta, accelerate the occurrence and development of AS.

关 键 词：动脉粥样硬化 炎症因子 miR-23a APOE-/-小鼠 

分 类 号：R543.3[医药卫生—心血管疾病] R332[医药卫生—内科学]