HPLC法测定肝舒乐颗粒中大黄素的含量  

Determination of Emodin in Ganshule Granule by HPLC

作　　者：胡安青 何寅 

机构地区：[1]江苏省镇江市药品检验所,镇江212002

出　　处：《北方药学》2017年第10期7-8,共2页Journal of North Pharmacy

摘　　要：目的:建立肝舒乐颗粒中大黄素含量的测定方法。方法:采用Thermoelectric C_(18)色谱柱;乙腈-甲醇-0.1%磷酸溶液(42∶23∶35)为流动相,流速:1.0m L·min-1;检测波长:254nm。结果:线性范围:大黄素2.025~30.381μg·m L^(-1)(r^2=0.999)。结论:该方法简便、专属性强,重复性好,可有效控制肝舒乐颗粒的质量。Objective： The method of determination of the Emodin in Ganshule Granule by HPLC was established The column was Thermoelectric C18 column,the mobile phase was aeetonitrile-methanol-0.1% phosphoric acid solution （42：23：35）. The flow rate was 1.0 mL·min^-1 and the detection wavelength was 254 nm. Reaults： The linear range of Emodin was2.025-30.381μg·mL^-1 （r^2=0.999）, the average recovery was 99.32%, RSD=0.4%（n=6）. Conclusions： This method is simple,rapid and accurate with good reproducibility, It is suitable for the quality control of Ganshule Granule processed.

关 键 词：肝舒乐颗粒 大黄素 HPLC 含量测定 

分 类 号：R286.0[医药卫生—中药学]