HPLC-MS/MS法测定人参制剂中Rg1、Rb1、Re、Rd的含量  

Detection of Ginsenoside Rg1,Rb1,Re,Rd in Preparations Using HPLC-MS/MS

作　　者：姚贞名 庄绪慧 刘文荣 倪莹莹 王文艳[1] YAO Zhen-ming ZHUANG Xu-hui LIU Wen-rong NI Ying-ying WANG Wen-yan(School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation （ Yantai University）, Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai 264005, Chin)

机构地区：[1]烟台大学新型制剂与生物技术药物研究山东省高校协同创新中心分子药理和药物评价教育部重点实验室(烟台大学),山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2017年第4期307-312,共6页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家自然科学基金资助项目(81102880);山东省优秀中青年科学家科研奖励基金项目(BS2011YY062)

摘　　要：建立液-质联用法以同时测定人参制剂中人参皂苷Rg1、Rb1、Re、Rd的含量.色谱柱为Waters symmetry C_(18)(150 mm×2.1 mm,3.5μm),流动相为乙腈-甲醇-水(50∶20∶30,含0.1%甲酸),流速为0.2 mL/min,柱温为30℃,采用正离子多反应监测模式检测.在本研究建立的实验条件下人参皂苷Rg1、Rb1、Re和Rd 4种成分线性关系良好,精密度、重复性、回收率的RSD均小于6%.本方法准确、灵敏、特异性好,流动相等度洗脱的条件下3.5 min之内便可完成4种皂苷的同时分离,适用于人参制剂中人参皂苷Rg1、Rb1、Re、Rd含量的同时测定.A sensitive and rapid liquid chromatography-tandem mass spectrometry method is developed to determine the content of ginsenoside Rg1, Rb1, Re, Rd in the ginseng preparation. The column of Waters symmetry C18 （ 150 mm× 2.1 mm, 3.5 μm） is employed for separation, the mobile phase is composed of 50% acatonitril, 20% methanol and 0.1% formic acid, and the flow rate is 0.2 mL/min. The data are acquired by the multiple reactions monitoring in the positive ion mode. In these chromatographic conditions, all the compounds show good linearity. The RSD of precision, repeatability and recovery in experiments are less than 6%. Four ginsenosides separation is achieved with the mobile phase isocratic elution in a total running time of 3.5 min. This method is proven to be specific, accurate, sensitive, and can be successfully applied to determine the contents of four ginsenoside in the ginseng preparations.

关 键 词：液相色谱质谱联用法 多反应监测 人参总皂苷 定量分析 

分 类 号：R917[医药卫生—药物分析学]