猴头菇DNA条形码的构建及验证  

Construction and Verification of DNA Barcode for Hericium erinaceus

作　　者：皇甫永冠 闫宝松[2] 张跃新[2] 宗宪春[3] Huangfu Yongguan Yan Baosong Zhang Yuexin Zong Xianchun(Northeast Forestry Universlty/Saline and alkaline land resources and Environment Research Center, Harbin 150040 Heilongjiang Forest Products Research Instituie, Heilongjiang Provincial Key Laboratory of Non-wood Forest Product Development,Mudanjiang, Heilongjiang 157011 Mudanjiang Normal University/College of life science and technology, Mudanjiang 157000)

机构地区：[1]东北林业大学盐碱地研究中心,哈尔滨150040 [2]黑龙江省林副特产研究所黑龙江省非木质林产品研发重点实验室,黑龙江牡丹江157011 [3]牡丹江师范学院,黑龙江牡丹江157000

出　　处：《中国林副特产》2017年第5期11-15,18,共6页Forest By-product and Speciality in China

基　　金：林业公益性行业科研专项经费(201404703-3)

摘　　要：为构建猴头菇品种专属的DNA条形码。应用分子生物学方法、选定β-tubulin基因作为条形码序列的目标基因,依据NCBI中猴头菇β-tubulin基因序列的全长,设计了一对用于扩增目标基因的引物:Betu-F1:ATGCGTGAAATCGTCCACC;Betu-R2:GAAGACGGGGGAAAGGAAC。应用上述引物对现有的19个猴头菇的品种进行PCR鉴定。测序后的序列结合BLAST、Clustal、MEGA、BioEdit等软件分析、比对得到多态性位点,分析多态性位点设计出构建条形码序列的特异性引物。经验证表明,猴头菇RT9品种的3个特有的条形码成功获得。试验结果表明,实验成功获得的DNA条形码序列可以将猴头菇RT9品种与猴头菇的其它品种区分鉴定。The aim was to construct the DNA barcode of Hericium erinaceus.Using molecular biology method,Selectedβ-tubulin gene as the target gene of bar code sequence,A pair of primers for amplification of the target gene was designed according to the full-length of theβ-tubulin gene sequence of NCBI：Betu-F1：ATGCGTGAAATCGTCCACC;Betu-R2：GAAGACGGGGGAAAGGAAC.The above primers were used to identify the 19 species of Hericium erinaceus of PCR.Sequencing of the sequence with BLAST,Clustal,MEGA,BioEdit and other software analysis,comparison of polymorphic loci,Analysis of polymorphic loci was designed to construct a specific primer sequence.The results showed that the three unique bar codes of Hericium erinaceus RT9 were successfully obtained.The experiment result shows：the DNA barcode sequence obtained from the experiment can distinguish the RT9 and other varieties of Hericium erinaceus

关 键 词：猴头菇 DNA条形码 内含子多态性 

分 类 号：S759.8[农业科学—森林经理学]