大鲵源浅黄金黄单胞菌的分离鉴定及药敏试验  

Isolationand identification of Pseudomonas Luteola from Giant Salamanderand theantibiotic sensitivitytest

作　　者：张海琳 罗杰[1] 吴青[2] 沙莎[1] ZHANG Hailin LUO Jie WU Qing SHA Sha(Department of Veterinary Medicine,Southwest University,Rongchang Campus,Department of Fisheries,Southwest University,Rongchang Campu)

机构地区：[1]西南大学荣昌校区动物医学系,重庆402460 [2]西南大学荣昌校区水产系,重庆402460

出　　处：《黑龙江畜牧兽医（下半月）》2017年第9期199-202,共4页

基　　金：国家"十二五"科技支撑计划项目"黑龙江等流域冷水鱼健康养殖关键技术继承与示范"(2012BAD25B10;2012BAD25B10-0);重庆市开州区农业委员会大鲵病害防控研究及技术服务采购项目(2015ZFCG150)

摘　　要：为确定导致重庆某大鲵人工养殖基地突发疾病的病原菌,试验从患病大鲵的肝脏分离出优势菌株(KN6-L211),通过人工感染鲫鱼进行致病性试验,采用菌落形态观察、生理生化特性分析、16S r DNA基因序列分析及常用药物的敏感性测定等方法鉴定该分离菌及其耐药状况,结果表明:该分离菌株KN6-L211为浅黄金黄单胞菌,该菌对鲫鱼具有很强的致病性,对头孢唑啉、头孢呋辛、头孢他啶、多西环素、氯霉素、吡哌酸、左氧氟沙星、四环素等8种药物高度敏感。To understand the pathogenic bacteria at a Giant Salamander farm in Chongqing,a predominant strain named KN6-L211 was isolated from the liver of diseased Giant Salamander. Based on pathogenic test by artificial infection of Crucian,colonial morphology,biochemical assays,16 S r DNA analysis and antibiotic sensitivity test,the isolates and their drug resistance were identified. The resultsindicatedthat KN6-L211 was identified as Pseudomonas Luteola,the strain had strong pathogenicity to crucian and high sensitivity to 8 kinds of antibiotics,including cefazolin,cefuroxime,ceftazidime,doxycycline,chloromycetin,pipemidic acid,levofloxacinand tetracycline.

关 键 词：大鲵 浅黄金黄单胞菌 分离鉴定 药敏试验 致病性 

分 类 号：S947.3[农业科学—水产养殖] S941.42[农业科学—水产科学]