L-谷氨酰胺通过p38 MAPK/cPLA2信号通路抑制变应性接触性皮炎  

Inhibition of allergic contact dermatitis by L-glutamine through p38 MAPK/cPLA2 signaling pathway

作　　者：仲卫明 金香[2] 郑明昱[2] 延光海[1] 金哲悟 ZHONG Weiming JIN Xiang ZHENG Mingyu YAN Guanghai JIN Zhewu(Department of Human Anatomy and Histoembryology, Yanbian University College of Medicine Department of Pediatrics of Chinese Medicine, Yanbian University College of Traditional Chinese Medicine ： Yanji 133002, Jilin , China Department of Anatomy, Jiangnan University College of Medicine, Wuxi 214122, Jiangsu , China)

机构地区：[1]延边大学医学院人体解剖学与组织胚胎学教研室 [2]延边大学中医学院中医儿科学,吉林延吉133002 [3]江南大学无锡医学院解剖学教研室,江苏无锡214122

出　　处：《延边大学医学学报》2017年第2期79-82,共4页Journal of Medical Science Yanbian University

基　　金：国家自然科学基金资助项目(81460471);吉林省科技发展计划项目重点项目(20140204046YY);吉林省教育厅(吉教科合字[2014]第16号)

摘　　要：[目的]探讨L-谷氨酰胺(Gln)对变应性接触性皮炎(ACD)模型小鼠的影响及其机制.[方法]利用2,4-二硝基氟苯反复刺激小鼠皮肤建立ACD模型,观察Gln对ACD模型小鼠耳肿胀度、组织病理改变、耳组织p38丝裂原活化蛋白激酶(MAPK)及胞浆型磷酯酶A2(cPLA2)蛋白表达和血清肿瘤坏死因子-α(TNF-α)及白细胞介素4(IL-4)等细胞因子表达的影响.[结果 ]与正常对照组比较,Gln显著抑制ACD模型小鼠耳肿胀度(P<0.05),明显降低皮肤组织p38 MAPK,cPLA2蛋白及血清TNF-α,IL-4炎性细胞因子表达水平(P<0.05).[结论]Gln治疗小鼠ACD有效,机制认为与p38 MAPK/cPLA2信号通路及TNF-α,IL-4炎性细胞因子表达有关系.OBJECTIVETo explore the effects of L-glutamine on mouse allergic contact dermatitis（ACD）and its mechanisms.METHODS The murine mode1 of ACD induced by 2,4-dinitrofluorobenzene（DNFB）was established.Ear swelling,histopathological changes and the protein expressions of p38 MAPK and cPLA2 in ear tissue were observed,the levels of tumor necrosis factorα（TNF-α）and IL-4 in serum were also determined.RESULTS As compared to the normal control group,L-glutamine could significantly inhibit mouse ear swelling（P〈0.05）,and down-regulate the expressions of p38 MAPK and cPLA2 in skin tissue and the serum levels of TNF-αand IL-4（P〈0.05）.CONCLUSION L-glutamine had therapeutic effects on mouse ACD,the mechanisms maybe associated with p38 MAPK/cPLA2 signaling pathway and the expressions of TNF-αand IL-4.

关 键 词：变应性接触性皮炎 L-谷氨酰胺 MAPK CPLA2 细胞因子 小鼠 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]