血清造血生长因子在宫颈癌早期诊断中的价值  

The early diagnostic utility of hematopoietic cytokines for cervical cancer

作　　者：邓凯贤[1] 郑玉华[1] 柳晓春[1] 汪洪[1] 胡路琴[1] 陈永连[1] 王玉玲[1] 黄晓斌[1] 李林夕 

机构地区：[1]佛山市妇幼保健院妇科,广东佛山528000

出　　处：《广东医学》2017年第19期2958-2960,共3页Guangdong Medical Journal

基　　金：广东省医学科研基金立项课题(编号:A2014703)

摘　　要：目的检测宫颈癌及癌前病变患者血清中造血生长因子(HGFs)的表达水平,评估HGFs在宫颈癌早期诊断中的应用价值。方法选择宫颈癌患者49例、宫颈上皮内瘤变(CIN)患者60例、健康体检者40例为研究对象,采取血清标本,酶联免疫法检测HGFs表达水平[包括干细胞因子(SCF)、粒细胞巨噬细胞刺激因子(GMCSF)、粒细胞集落刺激因子(G-CSF)和巨噬细胞集落刺激因子(M-CSF)],化学发光微粒免疫分析法检测宫颈癌肿瘤标记物鳞状细胞癌抗原(SCC-Ag)表达,对检测结果进行对比分析。结果 GM-CSF、G-CSF和M-CSF在宫颈癌及CIN患者中表达水平显著高于健康体检者(P<0.05),GM-CSF和M-CSF在宫颈癌患者的表达水平显著高于CIN患者(P<0.05),SCC-Ag在宫颈癌患者的表达水平显著高于CIN患者和健康体检者(P<0.05)。M-CSF、GM-CSF、G-CSF、SCC-Ag诊断宫颈癌的特异度均较高,其中M-CSF诊断的敏感度、阳性预测值和阴性预测值均高于SCC-Ag,M-CSF与SCC-Ag组合后诊断水平高于单独应用SCC-Ag。结论对HGFs的深入研究可能为寻找新的宫颈癌肿瘤标记物开辟新的途径,尤其是M-CSF具有作为一个良好的宫颈癌和CIN标记物的潜能,可作为早期诊断宫颈癌及CIN的可能因子。Objective To investigate the plasma levels of hematopoietic growth factors （HGFs） in patients with cervical cancer or cervical intraepithelial neoplasia （CIN） , thus to evaluate its application in early diagnosis of cervical cancer. Methods The plasma levels of HGFs （ SCF, GM - CSF, G - CSF and M - CSF） were determined by immunoenzyme assay （ EL1SA）, and SCC - Ag by chemiluminescent microparticle immunoassay. Results Significantly higher GM - CSF, G - CSF and M - CSF were observed in patients with cervical cancer and CIN than healthy controls （ P 〈 0. 05 ）. Significant higher GM - CSF and M - CSF were observed in patients with cervical cancer than those in patient with CIN （ P 〈 0. 05 ）. The diagnostic specificities GM - CSF, G - CSF, M - CSF and SCC - Ag were high for cervical cancer. The diagnostic sensitivity and the positive predictive value, and negative predictive value of M - CSF were higher than an- tigen SCCAg. Conclusion Further study of HGFs suggests new biomarker for cervical cancer. M - CSF can applied as a biomarker for early diagnosis of cervical cancer and CIN.

关 键 词：宫颈癌 宫颈上皮内瘤变 鳞状癌细胞抗原 造血生长因子 

分 类 号：R331.2[医药卫生—人体生理学]