高效液相色谱-高分辨质谱法快速筛查食品塑料包装袋中的5种苯并三唑类紫外吸收剂  

Rapid screening and detection of five benzotriazole ultraviolet stabilizers in food plastic packing bags by high performance liquid chromatography-orbitrap high resolution mass spectrometry

作　　者：勾新磊[1] 黄雯雯[1] 赵新颖[1,2] 高峡 张梅[1,2] 刘伟丽 Gou Xinlei Huang Wenwen Zhao Xinying Gao Xia Zhang Mei Liu Weili(Beijing Key Laboralory of Organic Materials Testing Technology＆ Quality Evaluation, Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China Beijing Engineering Research Center of Food Safety Analysis, Beijing 10089, China)

机构地区：[1]北京市理化分析测试中心有机材料检测技术与质量评价北京市重点实验室,北京100089 [2]北京市食品安全分析测试工程技术研究中心,北京100089

出　　处：《分析仪器》2017年第5期31-36,共6页Analytical Instrumentation

基　　金：北京市科学技术研究院创新工程Ⅲ-1(2015-178305);北京市科学技术研究院萌芽计划(2015-02)

摘　　要：建立了高效液相色谱-四极杆-静电场轨道阱高分辨质谱法快速筛查食品塑料包装袋中5种苯并三唑类紫外吸收剂的检测方法。样品使用甲醇-二氯甲烷混合溶剂超声萃取。用Waters ACQUITY UPLC BEH C18(100×2.1mm,1.7μm)色谱柱分离,甲醇和0.1%(v/v)甲酸水溶液为流动相进行梯度洗脱,通过高分辨质谱的全扫描/实时二级质谱扫描(Full Scan/ddMS2)模式进行检测,得到准确的母离子质量数和准确的碎片离子信息,构建了5种苯并三唑类化合物的筛查数据库,并进行定性定量分析。结果表明,5种目标物在1.0~100.0μg/L范围内线性关系良好,线性相关系数(R2)均大于0.994;定量限为0.5~2.5μg/kg;3个水平下的加标回收率为86.4%~97.5%,相对标准偏差为1.4%~7.2%。本方法简便、快速、可靠,专属性高,重现性良好,能满足食品塑料包装袋中5种苯并三唑类化合物的快速筛查与定量分析。The sample was ultrasonic extracted with methanol/dichloromethane （1 ： 1, v/v）. The separation was performed by water containing 0. 1 % （v/v） formic acid and methanol as the mobile phases with gradient elution at a flow rate of 0. 3 mL/min. Five benzotriazole ultraviolet stabilizers were screened qualitatively and determined quantitatively by full scan /ddMS2 mode. The standard curves of five compounds showed good correlation coefficients （R2〉0. 994） in their concentration range of 1.0-100.0μg/1. The limits of quantitation for five benzotriazole ultraviolet stabilizers were in the range of 0.5-2.5 μg/kg. The mean recoveries at three spiked levels （low, middle and high） were 86.4%- 97.5% with relative standard deviations of 1.4 %-7.2 %. This method is accurate, simple, rapid and feasible.

关 键 词：高效液相色谱 静电场轨道阱高分辨质谱 紫外吸收剂 苯并三唑类化合物 食品塑料包装袋 

分 类 号：O657.63[理学—分析化学] TS206.4[理学—化学]