养胃抗瘤冲剂对胃癌患者外周血T淋巴细胞rDNA的转录活性和凋亡相关蛋白影响的研究  

Effect of YangWeiKangLiu Granules on Peripheral Bood T-lymphocyte rDNA Transcription Activity and Fas Expression in Gastric Cancer Metastasis

作　　者：李杰[1] 孙桂芝[1] 祁鑫[1] 裴迎霞[1] 闫洪飞[1] 贺用和[1] 

机构地区：[1]中国中医研究院广安门医院,北京100053

出　　处：《中国中西医结合外科杂志》2002年第4期253-255,共3页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

摘　　要：目的 :观察养胃抗瘤冲剂对胃癌患者外周血T淋巴细胞rDNA转录活性和凋亡相关蛋白变化的影响及在肿瘤转移中的意义。 方法 :采用KL型肿瘤免疫图像分析系统和流式细胞仪测定正常人和胃癌患者养胃抗瘤冲剂治疗前后外周血T淋巴细胞rDNA的转录活性和调亡相关蛋白 (Fas/FasL)的表达变化。 结果 :胃癌患者外周血T淋巴细胞rDNA转录活性明显降低 ,凋亡相关蛋白Fas表达升高 ,与健康人相比 ,差异显著 (P <0 0 1) ,随着病情的进展 ,这种趋势更为明显。养胃抗瘤冲剂治疗后外周血T淋巴细胞的Ag -NORs升高、Fas表达降低 ,与治疗前比较差异显著 (P <0 0 1)。 结论 :养胃抗瘤冲剂可以降低外周血T淋巴细胞的凋亡能力 ,对增殖能力则有增强作用 ,从而提高机体的细胞免疫功能。外周血T淋巴细胞rDNA转录活性和凋亡相关蛋白变化可以从淋巴细胞增殖和凋亡活性两方面评价肿瘤患者的免疫状态 ,在胃癌转移的临床监测中具有重要意义。Objective:To evaluate the effect of YangWeiKangLiu granules on peripheral blood T lymphocyte cell rDNA transcription activity and Fas expression in gastric cancer metastasis Methods:A KL-2 imaging system was used to analyze the areas of Ag-NORs in T cells.The Fas expression in peripheral T-lymphocyte was analyzed by flow cytometry.Results:Treated by YangWeiKangLiu granules,the area of Ag-NORs was larger,and the Fas expression in peripheral T lymphocyte was lower,with significant differences( P <0 01).With development of the tumor,the Intension would be more obvious.Conclusion:The Fas expression on peripheral blood T lymphocyte was significantly lower and the area of Ag-NORs increased when treated by YangWeiKangLiu granules.The analysis of Ag-NORs and Fas Expression in T cells might play an important role in the monitoring of gastric cancer metastasis.

关 键 词：养胃抗瘤冲剂 胃癌 T淋巴细胞 RDNA转录活性 细胞凋亡 

分 类 号：R273.52[医药卫生—中西医结合] R283[医药卫生—中医肿瘤科]