养阴清热与润肺止咳验方防治放射性肺损伤的作用机制  

Mechanism of Yangyinqingre and Runfeizhike experiential prescription in the treatment of radiation induced lung injury

作　　者：黄常新[1] 鲍艳红 王婷 雷艳敏 李朝阳[1] 朱影[1] 姜贻乾[2] 

机构地区：[1]杭州师范大学附属医院肿瘤科,杭州310015 [2]杭州市萧山区第一人民医院放疗科,杭州311200 [3]杭州市余杭区良渚医院外科,杭州311112

出　　处：《中国临床药理学杂志》2017年第19期1922-1925,1941,共5页The Chinese Journal of Clinical Pharmacology

基　　金：浙江省重大科技专项基金资助项目(2017C03053);杭州市科技局基金资助项目(20140733Q18);杭州市卫生科技计划基金资助项目(2017B16)

摘　　要：目的研究养阴清热、润肺止咳验方防治放射性肺损伤的作用机制。方法用射线方法建立放射性肺损伤大鼠模型。将60只模型大鼠随机分为阳性对照组、模型组、实验A组、实验B组、实验C组,每组12只;另取,24只健康大鼠随机分为空白组和阴性对照组,每组12只。照射完成后次日,实验A、B、C组分别予以1,2,4 g·m L^(-1)养阴清热、润肺止咳验方;阳性对照组予以地塞米松;模型组和空白组均予以蒸馏水;阴性对照组予以2 g·mL^(-1)养阴清热、润肺止咳验方。7组大鼠均按0.01 m L·g^(-1)的剂量,每天灌服一次,总共14 d。14 d后处死大鼠,用Western blot法检测肺组织细胞中髓样分化蛋白88(My D88)、肿瘤坏死因子受体活化因子-6(TRAF-6)、诱导干扰素β的TIR基域接头分子(TRIF)、Toll样受体4(TLR4)的表达量,用酶联免疫吸附法检测肺组织匀浆上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-17(IL-17)、转化生长因子β(TGF-β)表达量。结果实验A、B、C组、模型组、阳性对照组、阴性对照组及空白组的My D88的灰度值(×10^(-2)))分别为(60.04±6.95),(51.75±6.50),(53.31±3.80),(63.27±0.06),(53.29±2.23),(21.43±5.42)和(21.16±4.81),TRAF-6的灰度值(×10^(-2)))分别为(77.44±4.16),(70.26±6.48),(66.05±4.17),(80.58±3.82),(65.23±2.60),(56.31±7.40)和(54.51±7.81),TRIF的灰度值(×10^(-2))分别为(48.01±6.51),(40.80±5.16),(41.20±5.18),(48.62±4.10,(39.44±4.25),(33.83±3.40和(32.49±4.71),TLR4的灰度值(×10^(-2)))分别为(65.13±4.53),(59.46±3.59),(57.13±4.83),(67.45±3.73),(56.65±3.82),(36.52±3.50)和(40.19±3.07),TNF-α分别为(255.71±4.87),(215.77±2.83),(211.27±3.81),(283.86±8.30),(208.21±1.31),(87.15±3.60)和(105.55±1.81)pg·m L^(-1),IL-17分别为(45.56±2.66),(32.84±3.64),(32.66±1.78,(48.56±1.26),(28.74±1.65),(17.26±3.00)和(20.44±2.69)pg·m L^(-1),TGF-β分别为(64.24±3.49),(53.19±4.30),(52.34±6.49),(67.09±6.04),(46.76±2.93),(38.22±6.16)和(38.37±1.74)ngObjective To explore the mechanism of Yangyinqingre andRunfeizhike experiential prescription in the treatment of radiation induced lung injury. Methods The model of radioactive lung injure rats was established by radiation. Sixty model rats were randomly divided into positive control group,model group,Test A group,Test B group,Test C group with 12 rats per group. Another 24 health rats were randomly divided into blank group and negative control group with 12 rats per group. At the next day after radiation,test A,B,C groups were given 1,2,4 g·m L^-1angyinqingre and Runfeizhike experiential prescription,positive control group was given dexamethasone,model and blank groups were given water,negative control group was given 2 g·m L^-1Yangyinqingre and Runfeizhike experiential prescription. The dose was 0. 01 m L·g^-1for 7 groups with qd for 14 days. after 14 d the rats were sacrificed,the expression of Myeloid differentiation factor 88（ My D88）,tumor necrosis factor receptor associated factor-6（ TRAF-6） and TIR domain containing adaptor inducing（ TRIF） and Toll-like receptor 4（ TLR4） were measured by Western blot method,the expression of tumor necrosis factor-α（ TNF-α）,interleukin-17（ IL-17） and transforming growth factor beta（ TGF-β） were measured by enzyme-linked immunosorbent assay（ ELISA）. Results The main indexes in test A,B,C groups,model group,positive control group,negative control group and blank group were compared： the gray value of My D88（ × 10^（-2））） were（ 60. 04 ± 6. 95）,（ 51. 75 ± 6. 50）,（ 53. 31 ±3. 80）,（ 63. 27 ± 0. 06）,（ 53. 29 ± 2. 23）,（ 21. 43 ± 5. 42） and（ 21. 16 ± 4. 81）,the gray value of TRAF-6（ × 10^（-2））） were（ 77. 44 ± 4. 16）,（ 70. 26 ± 6. 48）,（ 66. 05 ± 4. 17）,（ 80. 58 ± 3. 82）,（ 65. 23 ± 2. 60）,（ 56. 31 ± 7. 40） and（ 54. 51 ± 7. 81）,the gray value of TRIF（ × 10^（-2））） were（ 48. 01 ± 6. 51）,（ 40. 80 ± 5. 16）,（ 41. 20 ± 5. 18）,（ 48. 62

关 键 词：养阴清热与润肺止咳验方 放射性肺损伤 信号分子蛋白 细胞因子 

分 类 号：R28[医药卫生—中药学]