瑞芬太尼对急性重度失血性休克大鼠的肺保护作用研究  

Effect of lung protection of remifentanil in rats with acute severe hemorrhagic shock

作　　者：鲍传裕[1] 李龙鹤[1] 吴多虎[1] 邹永平[1] 毛健 

机构地区：[1]海南省人民医院急诊科,海口570311

出　　处：《中国临床药理学杂志》2017年第19期1926-1929,共4页The Chinese Journal of Clinical Pharmacology

基　　金：海南省哲学社会科学规划课题基金资助项目[HNS(YB)15-33];海南省卫生计生行业科研基金资助项目(14A200024)

摘　　要：目的研究瑞芬太尼对急性重度失血性休克大鼠的肺保护作用。方法选用50只健康清洁级成年SD大鼠,随机分为模型组,假手术组和低、中、高3个剂量实验组,每组10只。模型组建立急性重度失血性休克模型大鼠,其他组在模型组的基础上静脉注射负荷剂量瑞芬太尼1μg·kg^(-1),低、中、高3个剂量实验组分别以0.2,0.6,1.2μg·kg^(-1)·min^(-1)的速率静脉输注瑞芬太尼,假手术组游离但不阻断肺门,模型组与假手术组静脉输注与高剂量实验组等量的0.9%NaCl。病理检查判断大鼠肺组织损伤情况,测定各组肺组织干/湿重比值(W/D)、丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)含量。结果模型组W/D、MDA、SOD、NO分别为2.97±0.17,(3.45±1.48)nmol·mg^(-1) prot,(11.73±1.78)U·mg^(-1) prot,(0.13±0.03)μmol·mg^(-1) prot,低剂量实验组分别为2.71±0.12,(2.90±1.03)nmol·mg^(-1) prot,(14.44±1.55)U·mg^(-1) prot,(0.16±0.05)μmol·mg^(-1) prot,与模型组相比,差异均无统计学意义(均P>0.05)。中剂量实验组W/D、MDA、SOD、NO分别为2.33±0.16,(2.29±0.72)nmol·mg^(-1) prot,(15.59±1.80)U·mg^(-1) prot,(0.19±0.05)μmol·mg^(-1) prot,高剂量实验组分别为2.08±0.07,(1.81±0.64)nmol·mg^(-1) prot,(17.47±1.10)U·mg^(-1) prot,(0.26±0.08)μmol·mg^(-1) prot,与模型组比较,差异均有统计学意义(均P<0.05)。结论瑞芬太尼可通过抑制脂质过氧化,减轻肺组织损伤,对急性重度失血性休克大鼠的肺发挥保护作用。Objective To investigate the lung protection of remifentanil in rats with acute severe hemorrhagic shock（ ASHS）. Methods A total of 50 healthy adult SD rats were selected and randomly divided into model group,sham-operation group and low,middle,high dose test groups,10 rats in each group. Model group were ASHS model rats.Other groups were intravenous given injection with loading-dose of remifentanil 1 μg · kg^-1,then low,middle and high dose test groups were intravenous infusion with 0. 2,0. 6,1. 2 μg · kg^（-1）·min^-1 remifentanil. The lung hilus in model group and sham-operation group were dissociate without blockage, while model group and sham-operation group were infused with 0. 9% Na Cl as the same dose of high-dose test group. The injuries of lung tissues in rats were judged by pathological examination. The wet to dry weight ratio（ W/D） of lungtissue,malondialdehyde（ MDA）,activity of superoxide dismutase（ SOD）,content of nitrogen oxide（ NO） were detected in each group. Results The W/D,MDA,SOD,NO in model group were 2. 97 ± 0. 17,（ 3. 45 ± 1. 48）nmol·mg^-1 prot,（ 11. 73 ± 1. 78） U·mg^-1 prot,（ 0. 13 ± 0. 03） μmol·mg^-1 prot,had no significant difference with those in low dose test group, which were 2. 71 ± 0. 12,（ 2. 90 ± 1. 03） nmol · mg^-1 prot,（ 14. 44 ± 1. 55）U·mg^-1 prot,（ 0. 16 ± 0. 05） μmol·mg^-1 prot（ all P〈0. 05）. The W/D,MDA,SOD,NO in middle-dose test group were 2. 33 ± 0. 16,（ 2. 29 ± 0. 72） nmol · mg^-1 prot,（ 15. 59 ± 1. 80） U · mg^-1 prot,（ 0. 19 ± 0. 05）μmol·mg^-1 prot,and those in high-dose test group were 2. 08 ± 0. 07,（ 1. 81 ± 0. 64） nmol · mg^-1 prot,（ 17. 47 ± 1. 10） U·mg^-1 prot,（ 0. 26 ± 0. 08） μmol·mg^-1 prot,these indexes in middle-dose and high-dose test groups had significant difference with model group（ all P〈0. 05）. Conclusion Remifentanil can inhibit lipid peroxidation,reduce injury of lung tissue,so as to protect the lung of ASHS rats.

关 键 词：急性重度失血性休克 瑞芬太尼 肺保护 

分 类 号：R971.2[医药卫生—药品]