1-磷酸鞘氨醇受体1-3在Ⅰ型糖尿病ED大鼠阴茎海绵体组织中的表达  

Expressions of sphingosine-1-phosphate receptors 1-3 in the cavernous tissues of diabetes mellitus ED rats

作　　者：刘康 崔凯 李瑞 冯海航 刘继红[1] 文博[2,3] 饶可 Liu Kang Cui Kai Li Rui Feng Haihang Liu Jihong Wen Bo Rao Ke(Department of Urology, Tong/i Hospital, Tongji Medical College, I-Iuazhong University of Science and Technology Department of Urology, the People＇s Hospital of Baoan District, Southern Medical University Department of Urology, the People＇s Hospital of Shajing District, Guangzhou Medical University)

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030 [2]南方医科大学附属深圳宝安医院泌尿外科 [3]广州医科大学附属深圳沙井医院泌尿外科

出　　处：《中国男科学杂志》2017年第4期3-7,11,共6页Chinese Journal of Andrology

基　　金：深圳市科技计划项目(JCYJ20160427190559022)

摘　　要：目的探讨1-磷酸鞘氨醇受体1-3(S1PR1-3)在糖尿病性勃起功能障碍大鼠阴茎海绵体组织中的表达。方法 20只成年雄性SD大鼠随机分为正常对照组和糖尿病组,成功造模2个月后,测定两组大鼠阴茎海绵体内压/平均动脉压(Max ICP/MAP),采用Western blot分析S1PR1-3、eNOS、RhoA、ROCK1、ROCK2的表达。结果与对照组相比,糖尿病组大鼠勃起功能显著降低(P<0.05);糖尿病组大鼠阴茎海绵体S1PR1、S1PR3、eNOS蛋白表达水平下降(P<0.05),而S1PR2、RhoA、ROCK1、ROCK2的表达升高(P<0.05)。结论Ⅰ型糖尿病可导致大鼠勃起功能障碍,可能与S1PR1、S1PR3表达下调,eNOS/NO/cGMP信号通路受抑制以及S1PR2受体表达升高,激活RhoA/Rho激酶信号通路有关。Objective Abstract Objective To investigate the expressions of sphingosine-1-phosphate receptors 1-3 in the cavernous tissues of diabetes mellitus-induced erection dysfunction rats and its molecular mechanism. Methods Methods 20 eight-week-old healthy male SD rats were randomly divided into 2 groups： Control Group（CO, n=8） and Diabetes mellitus group（DM, n=12,）. Diabetes rats were firstly constructed. After 2 months, the maximum intracavernous pressure/mean arterial pressure（Max ICP/MAP） of rat models were determined and the expressions of S1PR1-3、eNOS、RhoA、ROCK1 and ROCK2 in the penis were detected by Western blot. Results Results The erectile function was significantly decreased in DM rats, compared to that of age-matched control rats（P 0.05）; the expressions of S1PR1、S1PR3 and eNOS were lower in group DM than those in group CO（P 0.05）, whereas the expressions of S1PR2、RhoA、ROCK1、ROCK2 were higher in group DM（P 0.05）. Conclusion Diabetes mellitus could result in significant reduction of Max ICP/MAP in male rats. Lower expressions of S1P1 and S1PR3 in the penis and inhibition of the eNOS/NO/c GMP signaling pathways, as well as higher expression of S1PR2 and activation of the RhoA/Rho kinase signaling pathways may contribute to this pathological process.

关 键 词：1-磷酸鞘氨醇受体 RHO相关激酶类 一氧化氮合酶 勃起功能障碍 糖尿病 

分 类 号：R698.1[医药卫生—泌尿科学] R587.1[医药卫生—外科学]