焦炉工GSTM1和GSTT1基因多态性与尿1-羟基芘浓度的关系研究  

Relationship between the GSTM1、GSTT1 gene polymorphism and 1-OHP concentration of the urine among the coke-oven workers

作　　者：漆骏[1] 李刚[1] 徐丹先[1] 何越峰 

机构地区：[1]云南省疾病预防控制中心,云南昆明650022 [2]昆明医科大学公共卫生学院,云南昆明650042

出　　处：《工业卫生与职业病》2017年第5期321-324,共4页Industrial Health and Occupational Diseases

基　　金：云南省科技厅资助项目(2009CD127)

摘　　要：目的探讨焦炉工GSTM1和GSTT1基因多态性与多环芳烃类(PAHs)代谢为1-羟基芘(1-OHP)过程之间的关系。方法以某焦化厂焦炉车间生产工人150人和其他车间无职业性PAHs接触者39人为调查对象。用高效液相色谱法测定尿中1-OHP浓度;聚合酶链反应一限制性片段长度多态性(PCRRFLP)法分别检测GSTM1和GSTT1基因多态性。采用方差分析和多因素回归分析等统计学方法研究影响尿中1-OHP浓度的因素。结果尿中1-OHP浓度与焦炉工作业的位置呈正相关(P<0.01)。低暴露组GSTT1(阴性)个体尿中1-OHP浓度较GSTT1(阳性)个体高;GSTM1(阴性)/GSTT1(阴性)全缺失型个体尿中1-OHP浓度明显高于GSTM1(阳性)/GSTT1(阳性)全野生型个体(P<0.05)。结论在PAHs低暴露水平下,GSTT1可以影响PAHs代谢。GSTM1和GSTT1基因的交互作用可以影响焦炉工PAHs代谢为1-OHP的过程。Objective To explore the relationship between the GSTM1、GSTT1 gene polymorphism and the process of the PAHs metabolize into 1-OHP. Methods 150 workers working in the coke-oven plants and 39 workers working in the other plants without PAHs exposure were recruited. High performed liquid chromatography（HPLC）methods were used to measure 1-OHP concentration of the urine. PCR-RFLP method were used to detect the GSTM1、 GSTT1 gene polymorphism. ANOVA and multiple factor regression analysis were used to explore the factors affecting the urinary concentration of 1-OHP. Results Urinary concentration of 1-OHP were positive related with the working position in the coke-oven. 1-OHP concentration of urine of GSTT1（negative）individuals were higher than GSTT1（positive）individuals in the low exposure group. 1-OHP concentration of urine of GSTT1（negative）/GSTM1（negative）individuals were higher than GSTT1（positive）/GSTM1（positive） individuals. Conclusions Under low exposure of the PAHs, GSTT1 polymorphism can affect the PAHs metabolism. GSTM1、 GSTT1 gene interaction can affect the process of PAHs metabolic into1-OHP.

关 键 词：焦炉工 1-羟基芘 GSTM1、GSTT1基因多态性 

分 类 号：R135.1[医药卫生—劳动卫生]