荧光定量PCR法与ELISA法联合检测乙型肝炎的临床价值探讨  

The study to the clinical value of fluorescence quantitative PCR method and ELISA method combined detection of hepatitis B

作　　者：高娟[1] 刘静[1] 

机构地区：[1]西安高新医院,陕西西安710075

出　　处：《中国优生与遗传杂志》2017年第9期29-31,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究荧光定量PCR法(FQ-PCR)检测乙肝病毒DNA(HBV-DNA)与ELISA法检测乙肝免疫学标志物(HBV-M)的临床价值。方法本研究选取2016年1月~2017年6月在西安高新医院检测的382例乙肝患者为研究对象,全部患者均采用荧光定量PCR法和ELISA法分别对HBV-DNA和乙肝两对半等免疫学指标进行检测,免疫学指标的检测顺序为乙型肝炎病毒表面抗原(HBs Ag)、乙肝病毒表面抗体(HBs Ab)、乙肝病毒e抗原(HBe Ag)、乙肝病毒e抗体(HBe Ab)、乙肝病毒核心抗体(HBc Ab)。比较不同HBV-M模式检测结果。结果模式1(大三阳组)HBV DNA阳性率最高,为94.6%,显著高于其他组,差异具有统计学意义(P<0.05)。模式1(大三阳组)的HBV DNA表达水平为(6.57±1.51)lg copies/ml,明显高于其他模式的HBV DNA表达水平,差异具有统计学意义(P<0.05)。结论采用FQ-PCR和HBV免疫学标志物检测相结合的方法,对临床乙型肝炎患者的早期诊断、病情判断、治疗方案的选择及预后具有重要指导价值。Objective：To investigate the clinical value of fluorescence quantitative PCR in detection of HBV DNA and ELISA in detection of HBV immunological markers. Methods：382 cases of hepatitis B patients in Xi an gao xin hospital were selected as the object of study from January 2016 to June 2017. The HBV-DNA and HBV immunological markers of all patients were detected by fluorescence quantitative PCR and ELISA respectively. The order of HBV immunological markers：Hepatitis B surface Antigen（HBs Ag）,Hepatitis B surface Antibody（HBs Ag）,Hepatitis B e Antigen（HBe Ag）,Hepatitis B e Antibody（HBeAb）,Hepatitis B core Antibody（HBc Ab）.Different HBV-M model test results were compared. Results：HBV-DNA positive rate of Pattern 1（HBe Ag positive patients）was 94.6%,which was significantly higher than that in other pattern and the difference was statistically significant（P〈 0.05）. HBV-DNA expression level of Pattern 1（HBe Ag positive patients）was which was（6.57±1.51）lg copies/ml,significantly higher than that in other pattern and the difference was statistically significant（P〈 0.05）. Conclusion：The combination of FQ-PCR and HBV immunological markers detection has an important guiding value for early diagnosis,treatment and prognosis of patients with hepatitis B.

关 键 词：荧光定量PCR HBV-DNA 乙肝免疫学标志物 酶联免疫吸附实验 

分 类 号：R512.62[医药卫生—内科学]